Team:BYU Provo/Team Thermosensor/Week22

Week 22 (Sep 11-17)

   ===9/12/11===
       *Plates moved
           - Streak plates moved from 30º to 37º @ 7:15 AM
               Blue colonies marked w/ a “B”
           - 63 2-11 Moved to 37º @ 7:25
           - 63-12, 13 and 19 are contaminated
           - 63 12-20 placed in 45º (42º?) in Grose’s lab @ 7:45
           - “Hot flash” plates not moved
       *Use red to mark the hours
       *Plates checked at 10:00 AM (Hour 2)
       *Plates checked at 12:00 (Hour 4) - Devin
       *Plates checked at 1:00 Hour 5 -Devin
       *Plates checked at 2:00 Hour 6 - Mark
           - Look at 5-1 at 3:00
       *Plates checked at 3:00 - Mark
           - 5-1 (in corner on racks) is starting to look blue
       *Checked at 4 PM - Chet hour 8
       *Checked at 5 PM - Mark hour 9
       *Checked at 6 PM - Chet hour 10
       *At 6 PM, plates 2-1 were placed in 42º incubator in Dr. Grose’s lab after being marked.
       *Marked with an “M” the colonies that are now blue in the 42º
       *Colonies 5-5-10-10 may have been on plates without enough x-gal in them or something, because not even the controls turned blue
   ===9/14/11===
       *Moved room temp plates to 42º 8:30 AM, leave in til 10 AM
       *Removed 37º-->42º plates
       *Plate 66-1 (hour 8 colonies)
           - Repeat check
           - 37º-->42º Start plates incubated at 37º then moved to 42º. Selecting colonies from original plates that were not blue at 37º but turned blue at 42º
       *66-2
           - Same as above but for 30º-->42º hours 4 and 5
       *66-3
           - Same as above but for hours 8-10
       *All left at room temp overnight
       *Plated all the old and new blue colonies from the plates that we watched change hour by hour
       *The colonies that changed from 37º-->42º are labeled 42-1A-->42-10A
           - “A” means they were from the new batch
       *Put in 30º incubator. Dr. Grose would like to see them before we start the next phase of the experiment