Team:BYU Provo/Team OxyR/Week16

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Team BYU Provo

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- Team OxyR:
  - The Beginning
  - Week 1
  - Week 2
  - Week 3
  - Week 4
  - Week 5
  - Week 6
  - Week 7
  - Week 8
  - Week 9
  - Week 10
  - Week 11
  - Week 12
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  - Week 14
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  - Week 16
  - Week 17
  - Week 18
  - Week 19
  - Week 20
  - Week 21
  - Week 22
  - Week 23
  - Week 24
  - Week 25
  - Week 26
  - Week 27
  - Week 28
- Team Thermosensor:
  - The Beginning
  - Week 1
  - Week 2
  - Week 3
  - Week 4
  - Week 5
  - Week 6
  - Week 7
  - Week 8
  - Week 9
  - Week 10
  - Week 11
  - Week 12
  - Week 13
  - Week 14
  - Week 15
  - Week 16
  - Week 17
  - Week 18
  - Week 19
  - Week 20
  - Week 21
  - Week 22
  - Week 23
  - Week 24
Indiana Trip
Outreach

1 August 2011

Mackay set up PCR to re-amplify OxyR-medium. Left note for Rob to run on gel when he came in next. Matt elaborated on the previous to-do list from July 28th:

List of plasmids and into which strains they go for electroporation:
- SoxRW (pIG24) into SoxR-
- SoxRM(pIG25) into SoxR-
- SoxRS(pIG26) into SoxR-
- HemH/OxyRW (pIG22) into OxyR-
- HemH/OxyRS (pIG23) into OxyR-
- KatG/OxyRW (pIG19) into OxyR-
- KatG/OxyRS (pIG20) into OxyR-
Plan plate reader protocol
Move (in database) IGM22-26 to plasmid section and rename to pIG22-26. Improve descriptions.

2 August 2011

Matt did electroporations with Dr. Grose using the newly-ordered SoxR- and OxyR- strains.

Diligent Julie, writing in the notebook.

3 August 2011

Matt made more to-do lists for mundane lab tasks such as making LB-amp plates, and stating the obvious such as "try ligations such-and-such over again". He also noted that from the previous day's electroporation attempt, all but two (IGM22 and 27) worked and gave colonies, though only IGM23's colonies turned green.

5 August 2011

Matt and Rob were up very early and in the lab by 7:00 am to perform the plate-reader experiment with the newly constructed plasmids, in their newly electroporated strains. Their hands were tired from so much pipetting, but they did manage to finish around 1:00 pm with some data in hand:

Plate Reader Experiment Results