Team:BYU Provo/Project

From 2011.igem.org

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{| class="TStable"
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! Part Number
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! Stable Temp.
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! Header 3
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! Melting Temp.
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| row 1, cell 1
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K619889 BBa_K619889]
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| 30'C
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| row 1, cell 3
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| 37'C
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| row 2, cell 1
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K619890 BBa_K619890]
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| row 2, cell 2
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| 35'C
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| row 2, cell 3
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| 37'C
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| row 3, cell 1
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K619891 BBa_K619891]
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| row 3, cell 2
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| 30'C
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| row 3, cell 3
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| 35'C
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|-
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| BBa_K619892
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| 35'C
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| 37'C
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|-
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| BBa_K619893
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| 35'C
 +
| 37'C
 +
|-
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| BBa_K619894
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| 35'C
 +
| 37'C
 +
|-
 +
| BBa_K619895
 +
| 35'C
 +
| 37'C
 +
|-
 +
| BBa_K619896
 +
| 35'C
 +
| 37'C
 +
|-
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| BBa_K619897
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| 35'C
 +
| 37'C
 +
|-
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| BBa_K619898
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| 35'C
 +
| 37'C
|}
|}

Revision as of 22:26, 28 September 2011

Team BYU Provo

BYU Provo
 

Contents

The Problem

X-Ray

The [http://www.cancer.org/Cancer/ColonandRectumCancer/DetailedGuide/colorectal-cancer-key-statistics American Cancer Society] has found that 1 in 20 Americans will have colorectal cancer. While current screening methods have led to a decrease in fatalities, the Society estimates another 50,000 people will die from colorectal cancers this year alone. As the disease progresses, it becomes [http://www.cancer.org/Cancer/ColonandRectumCancer/DetailedGuide/colorectal-cancer-survival-rates harder to successfully treat], making early detection important. Regular non-invasive screening could save tens of thousands of lives each year in the United States alone.


A Bioengineered Solution?

The Doctor
We think that E. coli could be engineered using simple interchangeable parts to act as an effective biosensor for colorectal cancer.

Dual-Input 'AND' Gate

We learned from the literature that polyps in the colon and rectum frequently have higher temperatures and higher concentrations of reactive oxygen species (ROS) than normal tissue. While either of these could potentially serve as an input to our biosensor, it is the elevation of both heat and ROS that makes polyps so unique in the human body. We have devised a simple molecular [http://en.wikipedia.org/wiki/AND_gate 'AND' gate] such that only in the elevated presence of both inputs will our marker protein be translated.

Visit our Modeling Page for more detailed information on the AND gate and how it works.

The Simple Interchangeable Parts

Please give the presentation thirty seconds to load after you press the 'play' button below. "Autoplay" can be selected under the "More" menu in the bottom right corner once the presentation is ready. Alternatively, the presentation is interactive and can be maneuvered with the mouse.

Final Construct on Prezi

Results

Part Number Stable Temp. Melting Temp.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K619889 BBa_K619889] 30'C 37'C
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K619890 BBa_K619890] 35'C 37'C
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K619891 BBa_K619891] 30'C 35'C
BBa_K619892 35'C 37'C
BBa_K619893 35'C 37'C
BBa_K619894 35'C 37'C
BBa_K619895 35'C 37'C
BBa_K619896 35'C 37'C
BBa_K619897 35'C 37'C
BBa_K619898 35'C 37'C



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