Team:BYU Provo/Team Thermosensor/Week22

From 2011.igem.org

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==Week 22 (Sep 11-17)==
==Week 22 (Sep 11-17)==
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    ===9/12/11===
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===9/12/11===
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        *Plates moved
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* Plates moved
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            - Streak plates moved from 30º to 37º @ 7:15 AM
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- Streak plates moved from 30º to 37º @ 7:15 AM; Blue colonies marked w/ a “B”
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                Blue colonies marked w/ a “B”
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- 63 2-11 Moved to 37º @ 7:25
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            - 63 2-11 Moved to 37º @ 7:25
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- 63-12, 13 and 19 are contaminated
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            - 63-12, 13 and 19 are contaminated
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- 63 12-20 placed in 45º (42º?) in Grose’s lab @ 7:45
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            - 63 12-20 placed in 45º (42º?) in Grose’s lab @ 7:45
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- “Hot flash” plates not moved
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            - “Hot flash” plates not moved
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* Use red to mark the hours
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        *Use red to mark the hours
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* Plates checked at 10:00 AM (Hour 2)
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        *Plates checked at 10:00 AM (Hour 2)
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* Plates checked at 12:00 (Hour 4) - Devin
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        *Plates checked at 12:00 (Hour 4) - Devin
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* Plates checked at 1:00 Hour 5 -Devin
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        *Plates checked at 1:00 Hour 5 -Devin
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* Plates checked at 2:00 Hour 6 - Mark
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        *Plates checked at 2:00 Hour 6 - Mark
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- Look at 5-1 at 3:00
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            - Look at 5-1 at 3:00
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* Plates checked at 3:00 - Mark
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        *Plates checked at 3:00 - Mark
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- 5-1 (in corner on racks) is starting to look blue
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            - 5-1 (in corner on racks) is starting to look blue
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* Checked at 4 PM - Chet hour 8
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        *Checked at 4 PM - Chet hour 8
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* Checked at 5 PM - Mark hour 9
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        *Checked at 5 PM - Mark hour 9
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* Checked at 6 PM - Chet hour 10
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        *Checked at 6 PM - Chet hour 10
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* At 6 PM, plates 2-1 were placed in 42º incubator in Dr. Grose’s lab after being marked.
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        *At 6 PM, plates 2-1 were placed in 42º incubator in Dr. Grose’s lab after being marked.
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* Marked with an “M” the colonies that are now blue in the 42º
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        *Marked with an “M” the colonies that are now blue in the 42º
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* Colonies 5-5-10-10 may have been on plates without enough x-gal in them or something, because not even the controls turned blue
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        *Colonies 5-5-10-10 may have been on plates without enough x-gal in them or something, because not even the controls turned blue
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===9/14/11===
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    ===9/14/11===
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* Moved room temp plates to 42º 8:30 AM, leave in til 10 AM
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        *Moved room temp plates to 42º 8:30 AM, leave in til 10 AM
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* Removed 37º-->42º plates
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        *Removed 37º-->42º plates
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* Plate 66-1 (hour 8 colonies)
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        *Plate 66-1 (hour 8 colonies)
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- Repeat check
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            - Repeat check
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- 37º-->42º Start plates incubated at 37º then moved to 42º. Selecting colonies from original plates that were not blue at 37º but turned blue at 42º
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            - 37º-->42º Start plates incubated at 37º then moved to 42º. Selecting colonies from original plates that were not blue at 37º but turned blue at 42º
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* 66-2
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        *66-2
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- Same as above but for 30º-->42º hours 4 and 5
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            - Same as above but for 30º-->42º hours 4 and 5
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* 66-3
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        *66-3
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- Same as above but for hours 8-10
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            - Same as above but for hours 8-10
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* All left at room temp overnight
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        *All left at room temp overnight
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* Plated all the old and new blue colonies from the plates that we watched change hour by hour
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        *Plated all the old and new blue colonies from the plates that we watched change hour by hour
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* The colonies that changed from 37º-->42º are labeled 42-1A-->42-10A
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        *The colonies that changed from 37º-->42º are labeled 42-1A-->42-10A
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- “A” means they were from the new batch
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            - “A” means they were from the new batch
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* Put in 30º incubator. Dr. Grose would like to see them before we start the next phase of the experiment
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        *Put in 30º incubator. Dr. Grose would like to see them before we start the next phase of the experiment
+
</div>
</div>

Revision as of 20:58, 28 September 2011

Team BYU Provo

BYU Provo
 

Week 22 (Sep 11-17)

9/12/11

  • Plates moved

- Streak plates moved from 30º to 37º @ 7:15 AM; Blue colonies marked w/ a “B” - 63 2-11 Moved to 37º @ 7:25 - 63-12, 13 and 19 are contaminated - 63 12-20 placed in 45º (42º?) in Grose’s lab @ 7:45 - “Hot flash” plates not moved

  • Use red to mark the hours
  • Plates checked at 10:00 AM (Hour 2)
  • Plates checked at 12:00 (Hour 4) - Devin
  • Plates checked at 1:00 Hour 5 -Devin
  • Plates checked at 2:00 Hour 6 - Mark

- Look at 5-1 at 3:00

  • Plates checked at 3:00 - Mark

- 5-1 (in corner on racks) is starting to look blue

  • Checked at 4 PM - Chet hour 8
  • Checked at 5 PM - Mark hour 9
  • Checked at 6 PM - Chet hour 10
  • At 6 PM, plates 2-1 were placed in 42º incubator in Dr. Grose’s lab after being marked.
  • Marked with an “M” the colonies that are now blue in the 42º
  • Colonies 5-5-10-10 may have been on plates without enough x-gal in them or something, because not even the controls turned blue

9/14/11

  • Moved room temp plates to 42º 8:30 AM, leave in til 10 AM
  • Removed 37º-->42º plates
  • Plate 66-1 (hour 8 colonies)

- Repeat check - 37º-->42º Start plates incubated at 37º then moved to 42º. Selecting colonies from original plates that were not blue at 37º but turned blue at 42º

  • 66-2

- Same as above but for 30º-->42º hours 4 and 5

  • 66-3

- Same as above but for hours 8-10

  • All left at room temp overnight
  • Plated all the old and new blue colonies from the plates that we watched change hour by hour
  • The colonies that changed from 37º-->42º are labeled 42-1A-->42-10A

- “A” means they were from the new batch

  • Put in 30º incubator. Dr. Grose would like to see them before we start the next phase of the experiment
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