Team:BYU Provo/Team Thermosensor/Week24

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Revision as of 02:23, 29 September 2011

Team BYU Provo

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Week 24 (Sep 25 - Oct 1)

9/26/11

4 gels checking cloning into iGEM parts plasmid

Gel 1

Gel1

Ladder, 8-21A, 8-21B, 8-21C, 8-21D, 8-21E, 5-13A, 5-13B, 5-13C, 6-3A, 6-3B, 6-3C, 6-3D, 6-3E Ladder, 6-3F, 6-3G, 6-3H, 4-13A, 4-13B, 4-13C, 4-13D, 4-13E, 4-13F, 4-13G, 4-13H, 5-1B

Gel 2

Gel2

Ladder, 5-1C, 5-1D, 5-3A, 5-3B, 5-3C, 5-3D, 10-1A, 10-1B, 10-1C, 10-1D, 10-1E, 10-1F, 10-1G Ladder, 10-1H, Empty, 5-12A, 5-12B, 5-12C, 5-12D, 5-12E, 5-12F, 5-12G, 5-12H, TSA-A, TSA-B, TSA-C

Gel 3

Gel3

Ladder, TSA-D, TSA-E, TSA-F, TSA-G, TSA-H, 8-76A, 8-76B, 8-76C, 8-76D, 8-76E, 8-76F, 8-76G, 8-76H Ladder, PCONA, PCONB, PCONC, PCOND, PCONE, PCONF, PCONG, PCONH, 10-8A, 10-8B, 10-8C, 10-8D, 10-8E, 10-8F, 10-8G, 10-9H

Gel 4

Gel4

Ladder, 5-12A, 5-12B, 5-12C, 5-12D, 5-12E, 10-4A, 10-4B, 8-28A Ladder, 5-1H, 5-1I, 5-1J, 5-1K, 5-1L, 5-1M, 5-1N, 5-1O, ControlX, ControlY

9/27/11

We mailed some of our parts to iGEM HQ. These are what they are named.

Our lab number --> iGEM part number
TSA (Wild type) --> BBa_K619889
10-1 --> BBa_K619890
6-3 --> BBa_K619891
8-21 --> BBa_K619892
5-3 --> BBa_K619893
10-8 --> BBa_K619894
4-3 --> BBa_K619895
pCon --> BBa_K619896

@@ Line 17: / Line 17: @@
 gels checking cloning into iGEM parts plasmid
-[[Image:PlasmidPartsBYUGel1.JPG|350px|Gel1]]
+==Gel 1==
-Ladder, 8-21A, 8-21B, 8-21C, 8-21D, 8-21E, 5-13A, 5-13B, 5-13C, 6-3A, 6-3B, 6-3C, 6-3D, 6-3E
+[[Image:Gel1.JPG|350px|Gel1]]
-Ladder, 6-3F, 6-3G, 6-3H, 4-13A, 4-13B, 4-13C, 4-13D, 4-13E, 4-13F, 4-13G, 4-13H, 5-1B
-[[Image:PlasmidPartsBYUGel2.JPG|350px|Gel2]]
+Ladder, '''8-21A,''' 8-21B, 8-21C, '''8-21D,''' 8-21E, '''5-13A,''' 5-13B, '''5-13C,''' 6-3A, 6-3B, '''6-3C,''' 6-3D, 6-3E
+Ladder, 6-3F, 6-3G, '''6-3H,''' 4-13A, 4-13B, 4-13C, 4-13D, 4-13E, 4-13F, 4-13G, 4-13H, 5-1B
-[[Image:PlasmidPartsBYUGel3.JPG|350px|Gel3]]
+==Gel 2==
+[[Image:Gel2.JPG|350px|Gel2]]
+Ladder, '''5-1C, 5-1D, 5-3A, 5-3B,''' 5-3C, 5-3D, '''10-1A, 10-1B,''' 10-1C, 10-1D, 10-1E, 10-1F, 10-1G
+Ladder, 10-1H, Empty, 5-12A, 5-12B, 5-12C, 5-12D, 5-12E, 5-12F, 5-12G, 5-12H, TSA-A, TSA-B, TSA-C
+==Gel 3==
+[[Image:Gel3.JPG|350px|Gel3]]
+Ladder, TSA-D, '''TSA-E, TSA-F,''' TSA-G, TSA-H, 8-76A, '''8-76B, 8-76C,''' 8-76D, 8-76E, 8-76F, 8-76G, 8-76H
+Ladder, '''PCONA, PCONB, PCONC, PCOND, PCONE, PCONF, PCONG, PCONH,''' 10-8A, 10-8B, '''10-8C,''' 10-8D, '''10-8E,''' 10-8F, 10-8G, 10-9H
+==Gel 4==
+[[Image:Gel4.JPG|350px|Gel4]]
+Ladder, '''5-12A,''' 5-12B, 5-12C, 5-12D, '''5-12E, 10-4A, 10-4B, 8-28A'''
+Ladder, 5-1H, 5-1I, 5-1J, 5-1K, 5-1L, 5-1M, 5-1N, 5-1O, ControlX, ControlY
-[[Image:PlasmidPartsBYUGel4.JPG|350px|Gel4]]
 ===9/27/11===