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Team:BYU Provo/Team OxyR/Week5
From 2011.igem.org
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==May 18== | ==May 18== | ||
Froze down bacterial strains: pGLOW, pPLAT, and pPLAT w/ GFP. | Froze down bacterial strains: pGLOW, pPLAT, and pPLAT w/ GFP. | ||
| + | Ligated above promoters into pPLAT w/ GFP and transformed them into DH5a E. Coli. | ||
| + | |||
| + | ==May 19== | ||
| + | Prepared colony PCR of above transformed bacteria to ensure the ligation and transformation worked. | ||
| + | |||
</div> | </div> | ||
Revision as of 18:08, 17 September 2011
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Contents |
May 16
Amplified OxyS, SoxS, KatG, and HemH promoters and cleaned up the products with ethanol.
May 17
Restriction digested the products from the previous day.
May 18
Froze down bacterial strains: pGLOW, pPLAT, and pPLAT w/ GFP. Ligated above promoters into pPLAT w/ GFP and transformed them into DH5a E. Coli.
May 19
Prepared colony PCR of above transformed bacteria to ensure the ligation and transformation worked.
