Team:BYU Provo/Team Thermosensor/Week22

Week 22 (Sep 11-17)

9/12/11

• Plates moved

Streak plates moved from 30º to 37º @ 7:15 AM; Blue colonies marked w/ a “B” 63 2-11 Moved to 37º @ 7:25 63-12, 13 and 19 are contaminated 63 12-20 placed in 45º (42º?) in Grose’s lab @ 7:45 “Hot flash” plates not moved

• Use red to mark the hours
• Plates checked at 10:00 AM (Hour 2)
• Plates checked at 12:00 (Hour 4) - Devin
• Plates checked at 1:00 Hour 5 -Devin
• Plates checked at 2:00 Hour 6 - Mark

Look at 5-1 at 3:00

• Plates checked at 3:00 - Mark

5-1 (in corner on racks) is starting to look blue

• Checked at 4 PM - Chet hour 8
• Checked at 5 PM - Mark hour 9
• Checked at 6 PM - Chet hour 10
• At 6 PM, plates 2-1 were placed in 42º incubator in Dr. Grose’s lab after being marked.
• Marked with an “M” the colonies that are now blue in the 42º
• Colonies 5-5-10-10 may have been on plates without enough x-gal in them or something, because not even the controls turned blue

9/14/11

• Moved room temp plates to 42º 8:30 AM, leave in til 10 AM
• Removed 37º-->42º plates
• Plate 66-1 (hour 8 colonies)

Repeat check 37º-->42º Start plates incubated at 37º then moved to 42º. Selecting colonies from original plates that were not blue at 37º but turned blue at 42º

• 66-2

Same as above but for 30º-->42º hours 4 and 5

• 66-3

Same as above but for hours 8-10

• All left at room temp overnight
• Plated all the old and new blue colonies from the plates that we watched change hour by hour
• The colonies that changed from 37º-->42º are labeled 42-1A-->42-10A

“A” means they were from the new batch

• Put in 30º incubator. Dr. Grose would like to see them before we start the next phase of the experiment

9/17/11

• Watched the plates for 10 hours and marked as the colonies changed from white to blue