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Team:BYU Provo/Team OxyR/Week10
From 2011.igem.org
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==June 20== | ==June 20== | ||
| + | [[File:PICT2423.JPG|200px|thumb|center|Results from plasmid purification.]] | ||
Purified plasmid from overnights containing SoxS and HemH with the weak, medium, and strong promoters for SoxR and OxyR, respectively. | Purified plasmid from overnights containing SoxS and HemH with the weak, medium, and strong promoters for SoxR and OxyR, respectively. | ||
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| + | ==June 22== | ||
| + | Did colony PCR to find OxyR medium and SoxS strong constructs. | ||
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| + | ==June 23== | ||
| + | Electroporation: plasmids with GFP only and GFP with the SoxS promoter into SoxR- strains; plasmids with GFP only and GFP with either the HemH and KatG promoters into OxyR- minus strains; plasmids with GFP only and GFP with one of the SoxS, HemH, and KatG promoters into wild-type ''E. Coli'' strains. | ||
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Latest revision as of 23:42, 27 September 2011
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June 20
Purified plasmid from overnights containing SoxS and HemH with the weak, medium, and strong promoters for SoxR and OxyR, respectively.
June 22
Did colony PCR to find OxyR medium and SoxS strong constructs.
June 23
Electroporation: plasmids with GFP only and GFP with the SoxS promoter into SoxR- strains; plasmids with GFP only and GFP with either the HemH and KatG promoters into OxyR- minus strains; plasmids with GFP only and GFP with one of the SoxS, HemH, and KatG promoters into wild-type E. Coli strains.
