Team:BYU Provo/Team OxyR/Week7

From 2011.igem.org

(Difference between revisions)
(May 31)
(June 1)
 
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Set up restriction digest and ligation for SoxS and HemH with the weak, medium, and strong promoters for the SoxR and OxyR genes, respectively
Set up restriction digest and ligation for SoxS and HemH with the weak, medium, and strong promoters for the SoxR and OxyR genes, respectively
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==June 3==
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Did another digest with identical to the one on June 1.
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Latest revision as of 23:06, 27 September 2011

Team BYU Provo

BYU Provo
 

May 31

Check to make sure the PCR clean up worked.

Purified OxyR and SoxS weak, medium, and strong promoters.

June 1

Sequencing results came back positive for SoxS and HemH promoters and negative for OxyS and KatG.

Set up restriction digest and ligation for SoxS and HemH with the weak, medium, and strong promoters for the SoxR and OxyR genes, respectively

June 3

Did another digest with identical to the one on June 1.