Team:BYU Provo/Team Thermosensor/Week5

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Template:BYU_Provo}} <html><script> var ele = document.getElementById('liTherm5'); ele.style.fontWeight = "bold"; ele.style.backgroundColor = "#059469"; jQuery(document...")
 
Line 11: Line 11:
<div id='pageContents'>
<div id='pageContents'>
 +
 +
==Week 5 (May 8-14)==
 +
 +
===5/20/11===
 +
 +
After the multiple failed attempts of amplifying LacZ we decided the error could no longer be our fault. We reordered primers, as it turns out sometimes the people who synthesize the primers make mistakes too.
 +
 +
Set up LacZ PCR using the recently ordered primers.
 +
* (19-1)
 +
* ~31 µl ddH20
 +
* 10 µl 5x phusion buffer
 +
* 1.5 µl 10mM dNTP’s
 +
* 1 µl primer IG39
 +
* 2 µl primer IG40
 +
* 1 µl diluted template DNA JHG526 (we renamed  pIGGY)
 +
* 0.5 µl phusion polymerase
</div>
</div>

Latest revision as of 02:27, 24 September 2011

Team BYU Provo

BYU Provo
 

Week 5 (May 8-14)

5/20/11

After the multiple failed attempts of amplifying LacZ we decided the error could no longer be our fault. We reordered primers, as it turns out sometimes the people who synthesize the primers make mistakes too.

Set up LacZ PCR using the recently ordered primers.

  • (19-1)
  • ~31 µl ddH20
  • 10 µl 5x phusion buffer
  • 1.5 µl 10mM dNTP’s
  • 1 µl primer IG39
  • 2 µl primer IG40
  • 1 µl diluted template DNA JHG526 (we renamed pIGGY)
  • 0.5 µl phusion polymerase
Retrieved from "http://2011.igem.org/Team:BYU_Provo/Team_Thermosensor/Week5"