Team:BYU Provo

From 2011.igem.org

(Difference between revisions)

Latest revision as of 18:23, 17 January 2012

Team BYU Provo

Home
Team
Project
Results
Protocols
Parts Submitted
Modeling
Safety
Attributions
Notebooks:
- Team OxyR:
  - The Beginning
  - Week 1
  - Week 2
  - Week 3
  - Week 4
  - Week 5
  - Week 6
  - Week 7
  - Week 8
  - Week 9
  - Week 10
  - Week 11
  - Week 12
  - Week 13
  - Week 14
  - Week 15
  - Week 16
  - Week 17
  - Week 18
  - Week 19
  - Week 20
  - Week 21
  - Week 22
  - Week 23
  - Week 24
  - Week 25
  - Week 26
  - Week 27
  - Week 28
- Team Thermosensor:
  - The Beginning
  - Week 1
  - Week 2
  - Week 3
  - Week 4
  - Week 5
  - Week 6
  - Week 7
  - Week 8
  - Week 9
  - Week 10
  - Week 11
  - Week 12
  - Week 13
  - Week 14
  - Week 15
  - Week 16
  - Week 17
  - Week 18
  - Week 19
  - Week 20
  - Week 21
  - Week 22
  - Week 23
  - Week 24
Indiana Trip
Outreach

About Us

In keeping with BYU's tradition of excellence, our team will represent BYU and all it stands for at the iGEM competition in Indiana, then Boston this year. This will be the first year that a team from BYU will be competing and we plan to demonstrate what Cougars are capable of.

Project Description

The International Genetically Engineered Machine competition (iGEM) is the world's premier synthetic biology competition. Last year 130+ teams from all continents participated. As BYU's first iGEM team, we propose constructing a molecular AND gate in E. coli. An AND gate requires two positive inputs to generate a positive output. To detect our chosen inputs we are investigating the OxyR promoter and a thermo-sensitive riboswitch. The OxyR protein activates transcription in the presence of hydrogen peroxide, a reactive oxygen species (ROS). The riboswitch allows translation only above a specific temperature. Both inputs activate a Cre/Lox system to remove a terminator sequence and allow transcription of a molecular signal. We intend to express this system in E. coli where, in the presence of both inputs, the cells will produce green fluorescent protein. A similar system, in theory, could be used for early detection of colorectal cancer.

Project Video

To learn more about the iGEM competition, visit this link: iGEM Website

@@ Line 1: / Line 1: @@
-[[File:BYU iGEM Logo.jpg|center|150px]]
+{{Template:BYU_Provo}}
-<center>
+<div id='pageContents'>
-<div style="padding: 10px; width: 730px">
+<html><script>
-<table class=menu>
+  var ele = document.getElementById('liHome');
-	<tr>
+  ele.style.fontWeight = "bold";
-	<td>
+  ele.style.backgroundColor = "#059469";
-	{| border=0  align="center" style="width:100%; color:#0000FF; background:#f1f5fc"
+</script></html>
-	! width="95px" | [[BYU iGEM|<font class=cell>'''Home'''</font>]]
-	! width="95px" | [[BYU iGEM/Contact|<font class=cell>'''Contact'''</font>]]
-	! width="95px" | [[BYU iGEM:Calendar|<font class=cell>'''Calendar'''</font>]]
-	! width="95px" | [[BYU iGEM/Papers|<font class=cell>'''Papers'''</font>]]
-	! width="95px" | [[BYU iGEM/Notebook|<font class=cell>'''Notebook'''</font>]]
-	! width="95px" | [[BYU iGEM/Team|<font class=cell>'''Team'''</font>]]
-	! width="95px" | [[BYU iGEM/Archive|<font class=cell>'''Archives'''</font>]]
-	|}
-	</td>
-	</tr>
-</table>
-<div style="width:719px;text-align:justify">
-[[File:BYU iGEM Logo.jpg|center|600px]]
 ==About Us==
-In keeping with BYU's tradition of excellence, our team will represent BYU and all it stands for at the iGEM competition in Boston next year.  This will be the first year that a team from BYU will be competing and we plan to demonstrate what Cougars are capable of.
-==About iGEM and Synthetic Biology==
+[[File:Doctor E.png|left|The Doctor]][[File:X ray E.png|right|X-Ray]]
-As a relatively young field of science, Synthetic Biology is proving to be of considerable interest.  Due to the nearly limitless diversification of life on earth, these organisms present a powerful framework for engineering complex chemical and biological processes.  Synthetic Biology takes advantage of the processes already extant within living things while seeking to use, biological, chemical and engineering principles to develop new processes to solve pressing issues.
+In keeping with BYU's tradition of excellence, our team will represent BYU and all it stands for at the iGEM competition in Indiana, then Boston this year.  This will be the first year that a team from BYU will be competing and we plan to demonstrate what Cougars are capable of.
+<br/><br/><br/><br/><br/>
+==Project Description==
-Some of the early achievements in Synthetic Biology are the engineering of E. coli to fluoresce different colors when exposed to UV light and bacterial production of human insulin.  Since then, researches in labs the world over have been laboring extensively to generate organisms capable of detecting soil composition for agriculture, discovering and producing better pharmaceuticals and even generating energy.  The ever-expanding potential of Synthetic Biology provides researchers the ability to work to solve real-world issues.
+The International Genetically Engineered Machine competition (iGEM) is the world's premier synthetic biology competition. Last year 130+ teams from all continents participated. As BYU's first iGEM team, we propose constructing a molecular AND gate in E. coli. An AND gate requires two positive inputs to generate a positive output.  To detect our chosen inputs we are investigating the OxyR promoter and a thermo-sensitive riboswitch. The OxyR protein activates transcription in the presence of hydrogen peroxide, a reactive oxygen species (ROS). The riboswitch allows translation only above a specific temperature. Both inputs activate a Cre/Lox system to remove a terminator sequence and allow transcription of a molecular signal. We intend to express this system in E. coli where, in the presence of both inputs, the cells will produce green fluorescent protein. A similar system, in theory, could be used for early detection of colorectal cancer.
-In 2003, the Massachusetts Institute of Technology (MIT) formed a class whose directive was to allow students to design their own biological systems.  The following year, MIT expanded the course into an intercollegiate competition now known as the International Genetically Engineered Machine or iGEM.
+==Project Video==
+<html><iframe width="560" height="345" src="http://www.youtube.com/embed/hkYrQJqXPEc" frameborder="0" allowfullscreen></iframe></html>
-One of the issues currently facing Synthetic Biology is the lack of standard parts in the synthesis of novel pathways.  The iGEM competition seeks to rectify this problem by developing a databank of standard parts.  These parts consist of genetic sequences that have proven useful in other Synthetic Biology projects.  By developing such a databank, much of the gene identification and isolation in a project may be eliminated, thus speeding along the completion of a project
-Over the years, this competition has grown substantially in participation and prestige. In 2010, iGEM welcomed 130 teams from all over the world to participate in the November conference by presenting their projects.
-To learn more about the iGEM competition, visit here: [https://igem.org/Main_Page iGEM Website]
+To learn more about the iGEM competition, visit this link: [https://igem.org/Main_Page iGEM Website] [[File:IGemGray.png|150px]]
+</div>