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Team:BYU Provo/Team Thermosensor/Week22
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| + | ==Week 22 (Sep 11-17)== | ||
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===9/12/11=== | ===9/12/11=== | ||
*Plates moved | *Plates moved | ||
Revision as of 20:54, 28 September 2011
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Week 22 (Sep 11-17)
===9/12/11===
*Plates moved
- Streak plates moved from 30º to 37º @ 7:15 AM
Blue colonies marked w/ a “B”
- 63 2-11 Moved to 37º @ 7:25
- 63-12, 13 and 19 are contaminated
- 63 12-20 placed in 45º (42º?) in Grose’s lab @ 7:45
- “Hot flash” plates not moved
*Use red to mark the hours
*Plates checked at 10:00 AM (Hour 2)
*Plates checked at 12:00 (Hour 4) - Devin
*Plates checked at 1:00 Hour 5 -Devin
*Plates checked at 2:00 Hour 6 - Mark
- Look at 5-1 at 3:00
*Plates checked at 3:00 - Mark
- 5-1 (in corner on racks) is starting to look blue
*Checked at 4 PM - Chet hour 8
*Checked at 5 PM - Mark hour 9
*Checked at 6 PM - Chet hour 10
*At 6 PM, plates 2-1 were placed in 42º incubator in Dr. Grose’s lab after being marked.
*Marked with an “M” the colonies that are now blue in the 42º
*Colonies 5-5-10-10 may have been on plates without enough x-gal in them or something, because not even the controls turned blue
===9/14/11===
*Moved room temp plates to 42º 8:30 AM, leave in til 10 AM
*Removed 37º-->42º plates
*Plate 66-1 (hour 8 colonies)
- Repeat check
- 37º-->42º Start plates incubated at 37º then moved to 42º. Selecting colonies from original plates that were not blue at 37º but turned blue at 42º
*66-2
- Same as above but for 30º-->42º hours 4 and 5
*66-3
- Same as above but for hours 8-10
*All left at room temp overnight
*Plated all the old and new blue colonies from the plates that we watched change hour by hour
*The colonies that changed from 37º-->42º are labeled 42-1A-->42-10A
- “A” means they were from the new batch
*Put in 30º incubator. Dr. Grose would like to see them before we start the next phase of the experiment
