Team:BYU Provo/Team Thermosensor/Week14

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==Week 14 (Jul. 17-23)==
 +
 +
===7/19/11===
 +
 +
Sequencing results back
 +
 +
pIG 15-IG40 (396bp)--aligned with lacZ from pcDNA3_lv5hislacz_seq
 +
 +
* score 250 bits
 +
* identities 164/188 (87%)
 +
* Strand plus/plus
 +
* E=4.8e^-70
 +
 +
pIG16-IG40 (640bp)--aligned with lacZ from pcDNA3_lv5hislacz_seq
 +
 +
* Score 1029 bits
 +
* E=0
 +
 +
pIG17-IG12 (963bp)--aligned with sfGFP sequence from Dr. Bundy
 +
 +
* No significant match
 +
 +
pIG 15 and pIG16 have inserted correctly! We can go ahead and move forward with those!
 +
 +
pIG17 & most likely pIG18 don’t have GFP
 +
 +
-Mark
 +
 +
===7/19/11===
 +
 +
Boiled template for LacZ∆TS (setting up to check for thermosensor insertion)
 +
 +
selected 8 colonies, diluted in 50microliters ddH20, then streaked on 8-slice plate (41-1). Also streaked for GFP∆TS (41-2)
 +
 +
-Julie
 +
 +
===7/20/11===
 +
 +
Standard Taq PCR set up as colony PCR for 8 colonies from plates 41-1 and 42-1.
 +
* Plate 41-1 colony PCR only because 41-2 colony PCR got “royally messed up” Not certain of the accuracy.
 +
 +
===7/21/11===
 +
 +
Remade boiled template so we can redo colony PCR if needed
 +
 +
* T41-1 boiled template M
 +
* T41-2 boiled template M
 +
 +
Ran Gel on previously set up colony PCR--there are two bands, one looks like possible thermosensor, the other is unknown
 +
 +
Made XGAL/ARA/AMP plates:
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 +
* 10g bacto-tryptone
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* 5g yeast
 +
* 5g NaCl
 +
* 5g Agar
 +
* .1g Arabinose
 +
* 1ml Amp
 +
* 1ml X-Gal
 +
 +
===7 July 2011===
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 +
Made LB/amp/ara/x-gal plates, put in fridge. Be CAEFUL: one bag has unlabeled plates. Label once dry.
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Latest revision as of 08:42, 24 September 2011

Team BYU Provo

BYU Provo
 

Contents

Week 14 (Jul. 17-23)

7/19/11

Sequencing results back

pIG 15-IG40 (396bp)--aligned with lacZ from pcDNA3_lv5hislacz_seq

  • score 250 bits
  • identities 164/188 (87%)
  • Strand plus/plus
  • E=4.8e^-70

pIG16-IG40 (640bp)--aligned with lacZ from pcDNA3_lv5hislacz_seq

  • Score 1029 bits
  • E=0

pIG17-IG12 (963bp)--aligned with sfGFP sequence from Dr. Bundy

  • No significant match

pIG 15 and pIG16 have inserted correctly! We can go ahead and move forward with those!

pIG17 & most likely pIG18 don’t have GFP

-Mark

7/19/11

Boiled template for LacZ∆TS (setting up to check for thermosensor insertion)

selected 8 colonies, diluted in 50microliters ddH20, then streaked on 8-slice plate (41-1). Also streaked for GFP∆TS (41-2)

-Julie

7/20/11

Standard Taq PCR set up as colony PCR for 8 colonies from plates 41-1 and 42-1.

  • Plate 41-1 colony PCR only because 41-2 colony PCR got “royally messed up” Not certain of the accuracy.

7/21/11

Remade boiled template so we can redo colony PCR if needed

  • T41-1 boiled template M
  • T41-2 boiled template M

Ran Gel on previously set up colony PCR--there are two bands, one looks like possible thermosensor, the other is unknown

Made XGAL/ARA/AMP plates:

  • 10g bacto-tryptone
  • 5g yeast
  • 5g NaCl
  • 5g Agar
  • .1g Arabinose
  • 1ml Amp
  • 1ml X-Gal

7 July 2011

Made LB/amp/ara/x-gal plates, put in fridge. Be CAEFUL: one bag has unlabeled plates. Label once dry.

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