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Team:BYU Provo/Team OxyR/Week16
From 2011.igem.org
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Matt elaborated on the previous to-do list from July 28th: | Matt elaborated on the previous to-do list from July 28th: | ||
# List of plasmids and into which strains they go for electroporation: | # List of plasmids and into which strains they go for electroporation: | ||
| - | + | #*SoxRW (pIG24) into SoxR- | |
| - | + | #*SoxRM(pIG25) into SoxR- | |
| - | + | #*SoxRS(pIG26) into SoxR- | |
| - | + | #*HemH/OxyRW (pIG22) into OxyR- | |
| - | + | #*HemH/OxyRS (pIG23) into OxyR- | |
| - | + | #*KatG/OxyRW (pIG19) into OxyR- | |
| - | + | #*KatG/OxyRS (pIG20) into OxyR- | |
# Plan plate reader protocol | # Plan plate reader protocol | ||
| - | # Move (in database) IGM22-26 to plasmid section and rename to pIG22-26. Improve descriptions. | + | # Move (in database) IGM22-26 to plasmid section and rename to pIG22-26. Improve descriptions. |
==2 August 2011== | ==2 August 2011== | ||
Revision as of 05:04, 14 September 2011
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Contents |
1 August 2011
Mackay set up PCR to re-amplify OxyR-medium. Left note for Rob to run on gel when he came in next. Matt elaborated on the previous to-do list from July 28th:
- List of plasmids and into which strains they go for electroporation:
- SoxRW (pIG24) into SoxR-
- SoxRM(pIG25) into SoxR-
- SoxRS(pIG26) into SoxR-
- HemH/OxyRW (pIG22) into OxyR-
- HemH/OxyRS (pIG23) into OxyR-
- KatG/OxyRW (pIG19) into OxyR-
- KatG/OxyRS (pIG20) into OxyR-
- Plan plate reader protocol
- Move (in database) IGM22-26 to plasmid section and rename to pIG22-26. Improve descriptions.
2 August 2011
Matt did electroporations with Dr. Grose using the newly-ordered SoxR- and OxyR- strains.
3 August 2011
Matt made more to-do lists for mundane lab tasks such as making LB-amp plates, and stating the obvious such as "try ligations such-and-such over again". He also noted that from the previous day's electroporation attempt, all but two (IGM22 and 27) worked and gave colonies, though only IGM23's colonies turned green.
5 August 2011
Matt and Rob were up very early and in the lab by 7:00 am to perform the plate-reader experiment with the newly constructed plasmids, in their newly electroporated strains. Their hands were tired from so much pipetting, but they did manage to finish around 1:00 pm with some data in hand:
