The following standard protocols were used during our lab work: [[Media:Freiburg11_Transformaion.pdf|Transformation]], [[Media:Freiburg11_Digestion.pdf|Digestion]], [[Media:Freiburg11_Gibson-Assembly.pdf|Gibson-Assembly]], [[Media:Freiburg11_Geldocumentation.pdf|Gel documentation]], [[Media:Freiburg11_Gelextraction.pdf|Gel extraction]], [[Media:Freiburg11_Ligation.pdf|Ligation]], [[Media:Freiburg11_PCR.pdf|PCR]], [[Media:Freiburg11_Plasmidminprep.pdf|Plasmid-Miniprep]], [[Media:Freiburg11_Testdigest.pdf|Testdigest]], [[Media:Freiburg11_GST purification.pdf|GST-Purification]], [[Media: Freiburg11_Bradford assay.pdf|Bradford assay]].
These primer and strains were used: [[Media:Freiburg11_Primerlist.pdf|Primerlist]], [[Media:Freiburg11_Straincollection.pdf|Straincollection]].
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