Sequencing
Primer concentration has to be 2 nM.
Dilution of stock: 2 μl in 100 μl H2O.
max 100 ng/μl of DNA.
pos. clone
| ng/μl
| μl
|
PR1e
| 46.8
| 2.1
|
PR2d
| 67.9
| 1.4
|
PR3e
| 45.8
| 2.2
|
PR4b
| 61.5
| 1.6
|
PR5a
| 74.5
| 1.3
|
PR6c
| 94.7
| 1.06
|
PR7b
| 69.1
| 1.4
|
PR8b
| 81.9
| 1.2
|
PR9a
| 64.4
| 1.5
|
lys 1
| 16
| 10
|
lys 2
| 21.7
| 10
|
lys 3
| 2.5
| 10
|
lys 4
| 16.4
| 10
|
lys 5
| 10.2
| 10
|