Team:Freiburg/Notebook/25 August

• < home >
• < team >
  • → members
  • → attributions
  • → freiburg
• < project >
  • → description
  • → results
  • → modeling
  • → notebook
  • → sample data
• < human practices >
  • → oath
  • → ethics
  • → safety
• < gallery >
• < sponsors >
• < to the forums >

Commons

PCR of pSB1A3 and pSB1C3

Investigators: Sandra

Primer:

• P35
• P36

Testdigest:

Expected bands:
pSB1A3: 2155 bp

pSB1C3: 2070 bp

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

Miniprep

Investigators: Sandra

Miniprep of LovTAP-NotGate-pSB1A3.

After Miniprep the samples were digested with:

• EcoRI
• PstI

The testdigest showed no bands!

Trafo

Investigators: Sandra

Transformation of LovTAP-NotGate-pSB1A3 again, because the last transformation did not show good colonies to pick. Incubation over night.

Clones need to be picked tomorrow.

3A-assembly:Digestion

Investigators: Sandra

Digestion of:

• Not
• nOt
• noT
• Lovtap 3A
• pSB1A3

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

Insertion of the precipitator into a iGEM vector

Investigators: Julia
The precipitator was cloned into pSB1K3 and pSB1T3.

Miniprep

Investigators: Sandra
continue from Transformation
22.08.11
Investigator: Sophie
Project name: inducible promoter for pbd

Testdigest

Investigators: Sophie
continue from Miniprep
Investigatora: Sandra
project name: inducible promoter for pbd