Precipitator
Protein modelling with pymol
Investigators: Rüdiger
Amplification of different parts
Investigators: Julia and Jacob
antibiotics plates:
1oo mg/ml ampicillin (amp) (100 microliter per plate)
1oo mikroliter H2O + 2 microliter canamycin (cm)
1oo mikroliter H2O + 40 microliter tetracyclin(tet)
1oo mikroliter H2O + 10 microliter kanamycin (kn)
| part
| location(p=plate)
| resistance
| info
|
| BBa_K098995
| P3, 1E
| amp
| heat sensitive cI QPI with high promoter
|
| BBa_K112022
| P3, 24E
| amp
| Lambda phage lysis device - no promoter, This part is in BBb Format.
It is flanked by BamHI and BglII sites instead of XbaI and SpeI.
|
| pSB1K3
| P1, 5A
| kan
| high copy BioBrick assemby plasmid
|
| pSB1A3
| P1, 1G
| amp
| high copy BioBrick assemby plasmid
|
| pSB1C3
| P1,3A
| cm
| high copy BioBrick assemby plasmid
|
| pSB1T3
| P1, 7A
| tet
| high copy BioBrick assemby plasmid
|
| J23104
| P1, 18K
|
| promotor
|
| J23110
| P1, 20 C
|
| promotor
|
| J23116
| P1, 20M
|
| promotor
|
| B0034
| P1, 2M
|
| RBS (strong)
|
| B0032
| P1, 2 I
|
| RBS.3 (medium)
|
| B0031
| P1, 2G
|
| RBS.2 (weak)
|
Transformation of parts in competent cells and plating them out on plates with specific antiobiotic resistance.
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