Team:Freiburg/Notebook/17 June

From 2011.igem.org

This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

1 green light receptor
- 1.1 PCR with Ccar and Ccas
2 Lysis cassette
- 2.1 3A assembly: Digestion
- 2.2 Miniprep

green light receptor

PCR with Ccar and Ccas

Investigators: Julia

different primer used for PCR:

	Primer
Ccas	5 and 7
Ccar	4 and 6

Lysis cassette

3A assembly: Digestion

Investigators: Theo

Vector: Backbone chloramphenicol
partI: S11
partII: S15

	Enzyme 1	Enzyme 2
Vector	EcoRI	PstI
PartI	EcoRI	SpeI
PartII	Xbal	PstI

Miniprep

Investigators: Theo

Minipreps of BBa_E0040, BBa_K124014, BBa_M30109

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