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Lysis cassette

3A assembly: Ligation

Investigators: Theo

Comments: Ligation of digested parts from yesterday.

S11: heat sensitive cI QPI with a high promoter preceding the repressor
S15: Bacteriophage Holin Gene pS105
S1, S2, S3: promotor
S4, S5, S6: RBS
Target vector

Transformation

Investigators: Theo

Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor.