green light receptor
1. Quickchange
Investigator:Jakob
| Name:
Jakob
| Date:
08.08.2011
|
| Continue from Experiment:
|
| Project Name:
Quickchange CcaR and CcaS
|
PCR-Mixture for one Reaction:
For a 50 µl reaction use
| 32,5µl
| H20
|
|
| 10µl
| 5x Phusion Buffer
|
|
| 2.5µl
| Primer fw
| Primer: P12(CcaR) and P14(CcaS
|
| 2.5µl
| Primer dw
| Primer: P13(CcaR) and P15(CcaS)
|
| 1µl
| dNTPs
|
|
| 1µl
| DNA-Template
| DNA: CcaR and CcaS
|
| 0.5 µl
| Phusion (add in the end)
|
|
2. Digest with DpnI
- Digest of the quickchange above
3. Transformation
- Transformation from digest above
- Note: I'm such a fool...forgot the gel...
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
Lysis + RBS
Investigators:Theo
Sent samples for sequencing, Testdigestions of whole temperature sensitive cassette showed no correct inserts! (:()
Precipitator
NAME OF YOUR EXPERIMENT: Cloning
Investigators: Sophie
continue from experiment: miniprep (the, 8.8.)
Project name: new 3A assembly with amp vector
Parts: psB1A3 (Eco & Pst)
GFP-pbd ( Xba & Pst)
PR4 or PR6 (Eco & Spe)
Why? The last cloning of Theo and Jakob produced one clone with GFP-pbd inside but without PR. So I'm trying again to clone with two different PRs with different RBS strenghts.
Stored in: "Minipreps, verdaut"-box
"
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