green light receptor
Results from the Transformation
Investigators: Jakob
Results are again negativ...
- To-do: Tomorrow we will have a call with the iGEM-Team from Uppsala Sweden, maybe they can give us some favour.
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
3A-assembly
Investigators: Sandra
Digestion
- PR1 (pSB1C3)
- PR2 (pSB1C3)
- pcyA (pSB1T3)
- pcyATd (pSB1T3)
- pcyATb (pSB1T3)
- L23 (pSB1T3)
- vector (pSB1K3)
Amount of DNA and H20:
| Sample
| 500(ng)/DNA concentration (ng/μl)
| H20 μl
|
| pcyATd
| 2.8
| 35.2
|
| pcyaTb
| 2.9
| 35.1
|
| PR2
| 7.2
| 30.8
|
| PR1
| 6.1
| 31.9
|
| L23
| 4.8
| 33.2
|
| pSB1K3
| 20
| 18
|
Enzymes necessary for digestion:
|
| PR1/PR2
| pcyA/L23
| vector
|
| enzyme 1
| EcoRI
| XbaI
| EcoRI
|
| enzyme 2
| SpeI
| PstI
| PstI
|
-incubated for 1 hours at 37°C.
-heated for 20 minutes at 80°C
Ligation
Ligation of:
|
| PcayTd
| PcyATb
| L23 (ho1)
|
| PR1
| 1 pTd
| 1 pTb
| 1 L23
|
| PR2
| 2 pTd
| 2 pTb
| 2 L23
|
Named the samples, see above in table.
Transformation
Transformation of ligated part:
- 1pTd (PR1-pcyATd)
- 2pTd (PR2-pcyATd)
- 1pTb (PR1-pcyATb)
- 2pTb (PR2-pcyATb)
- 1L23 (PR1-Ho1T)
- 2L23 (PR2-Ho1T)
plated out on plates with kanamycin resistance.
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Trafo
Investigators: Sophie
Trafo of new 3A-assembly parts in amp vector.
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