Team:UNICAMP-EMSE Brazil/Notebook/24 August 2011

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(Created page with "{{Template:UNICAMP-EMSE Brazil_Notebook}} <div style="text-align: left;"> [[Image:UNICAMP-EMSE_Brazil_Gel1_24_08_11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel...")
 
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'''Result:'''
'''Result:'''
*Very low concentration! New digestion to do with all the remaining from the Miniprep…
*Very low concentration! New digestion to do with all the remaining from the Miniprep…
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Q[0] = "<i>I’m Luke Skywalker, I’m here to rescue you.</i>Luke, Star Wars: Episode IV-A New Hope (1977)"
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Q[1] = "<i>Watch your mouth kid, or you’ll find yourself floating home.</i>Star Wars: Episode IV-A New Hope (1977)"
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Q[2] = "<i>Evacuate in our moment of triumph? I think you overestimate their chances.</i> Star Wars: Episode IV-A New Hope (1977)"
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Q[3] = "<i>The Force is strong with this one.</i> Star Wars: Episode IV-A New Hope (1977)"
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Q[4] = "<i> I have you now!</i> Darth Vader, Star Wars: Episode IV-A New Hope (1977)"
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Q[5] = "<i> Fear is the path to the dark side. Fear leads to anger, anger leads to hate, hate leads to suffering.</i> MsC Yoda, Star Wars Episode I: The Phantom Menace (1999)"
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Q[6] = "<i> No blasters! No blasters!.</i> A bartender, Star Wars: Episode IV-A New Hope (1977)"
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Q[7] = "<i> Go to the center of the gravity's pull, and find your planet you will...</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[8] = "<i> Clear, your mind must be if you are to discover the real villains behind the plot.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[9] = "<i> Much to learn, you still have.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[10] = "<i> Meditate on this, I will.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[11] = "<i> Use the Force, Luke.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[12] = "<i> May the Force be with you.</i> Mentioned several times throughout the Star Wars saga"
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Q[13] = "<i> I really need a Jedi Bacteria.</i> Iolanda Albuquerque, UNICAMP-EMSE team advisor"
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Q[14] = "<i> Another splendid day in Brazil!!!</i> Marc Emery, UNICAMP-EMSE team student"
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Q[15] = "<i> Dont' cut, let your hair volume expressing all their feelings...</i> Thibault Sabattier (UNICAMP-EMSE team student) about Nemailla's hair"
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Q[16] = "<i> What a good life!!</i> Louise Marais (UNICAMP-EMSE team student) about living in Brazil"
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Q[17] = "<i> Gosh!I will have nigthmares tonight! </i> Danieli Gonçalves (UNICAMP-EMSE team student) about Thibault wearing Marianna's dress"
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Q[18] = "<i> I will revenge myself against you and Marc Emery!!!</i> Nemailla Bonturi (UNICAMP-EMSE team advisor)"
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Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses"
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Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)"
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index = Math.floor(Math.random() * Q.length);
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document.writeln(Q[index]);
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Latest revision as of 00:15, 30 August 2011

Home Project Methods Results Data Team Notebook Human Practices Safety Profile Sponsors Wix

Click here to see our PROTOCOLS

Contents

Notebook

Click on a date to see what we have done!

May
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
June
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
July
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
August
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31


Electrophoresis Gel 1, 24/08/2011.
Gel 1:: HLyA / RBS+HLyD sample 1 / RBS+HLyD sample 2.

24 August 2011

New analysis of HlyA and HlyD

Objective: Quantification of HLyA and RBS+HLyD (volume of ladder applied 6 uL; sample: 5 uL DNA +1 uL buffer )

  • Ladder: Bio-Rad 100bp – 10.000bp
  • Samples:
  • HLyA / RBS+HLyD sample 1 / RBS+HLyD sample 2


  • Results:

Gene Total size (gene+vector) Linear vector size Gene size Result
HlyA 3027 pb 2800 pb 227 pb Ran out of the gel…
RBS+HlyD 4296 pb 2800 pb 1496 pb Ok, sample 2 better than 1



-All the genes were purified using Fermentas kit.


Quantification of HlyA

Electrophoresis Gel 2, 23/08/2011.
Ladder and HLyA

Objective: quantification of HLyA (again!)

  • Samples:
  • Ladder and HlyA


Result:

  • Very low concentration! New digestion to do with all the remaining from the Miniprep…






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