Team:UNICAMP-EMSE Brazil/Notebook/10 September 2011
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10 September 2011
Quantification electrophoresis
Objective:
- Quantify the purified genes concentration.
Samples:
- Gel 1:
- Const promoter+RBS+SoxR+T / SoxS+RBS+GFP+HlyA+T
- Ladder:
- 100-10.000 bp (Fermentas)
- Gel Agarose concentration:
- 1,0%
- Results:
Construction | Total size (gene+vector) | Linear vector size | Gene size | Result |
---|---|---|---|---|
Const promo+ RBS+SoxR+T | ~3850 bp | ~3300 bp | 558 bp | Very low concentration: less than 4ng/ul ? |
SoxS+RBS+GFP+HlyA+T | 4365 bp | 3205 bp | 1160 bp | Very low concentration: less than 4ng/ul ? |
OPS... The sizes don’t seem to match...