Team:UNICAMP-EMSE Brazil/Notebook/10 September 2011

From 2011.igem.org

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Notebook

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May
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
June
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
July
MTWTFSS
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4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
August
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
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31


Electrophoresis Gel 1, 10/09/2011.
Gel 1: Const promo+ RBS+SoxR+T ; SoxS+RBS+GFP+HlyA+T

10 September 2011

Quantification electrophoresis

Objective:

  • Quantify the purified genes concentration.

Samples:

  • Gel 1:
  • Const promoter+RBS+SoxR+T / SoxS+RBS+GFP+HlyA+T
  • Ladder:
  • 100-10.000 bp (Fermentas)
  • Gel Agarose concentration:
  • 1,0%


  • Results:

Construction Total size (gene+vector) Linear vector size Gene size Result
Const promo+ RBS+SoxR+T ~3850 bp ~3300 bp 558 bp Very low concentration:
less than 4ng/ul ?
SoxS+RBS+GFP+HlyA+T 4365 bp 3205 bp 1160 bp Very low concentration:
less than 4ng/ul ?




OPS... The sizes don’t seem to match...