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Team:UNICAMP-EMSE Brazil/Notebook/19 August 2011
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19 August 2011
Synthetic DNA sequences status
Objective: Check the size of synthetic DNA sequences.
- Ladder: 100-10.000 bp (Fermentas)
- Agarose concentration: 0.8%
- Samples:
- GEL 1:
- 1. Const. Promoter1 (nd)
- 2. Const. Promoter1
- 3. flhDC1 (nd)
- 4. flhDC1
- 5. flhDC2
- 6. QseB-QseC1 (nd)
- 7. QseB-QseC1
- 8. RBS-SoxR 1 (nd)
- 9. RBS-SoxR 1
- 10. RBS-IL10 1 (nd)
- 11. RBS-IL10 1
- 12. RBS-IL10 2
- 13. RBS-IL12 1 (nd)
- 14. RBS-IL12 1
- 15. RBS-IL12 2
- 16. Ladder
- GEL2:
- 17. Ladder
- 18. RBS-HylB 1 (nd)
- 19. RBS-HylB 1
- 20. RBS-HylB 2
- 21. HylA 1 (nd)
- 22. HylA 1
- 23. HylA 2
- 24. RBS-HylD 1 (nd)
- 25. RBS-HylD 1
- 26. RBS-HylD 2
- Results:
| Gene | Total size (gene+vector) | Linear vector size | Gene size | Result |
|---|---|---|---|---|
| Constitut. promoter | 2795 | 2760 | 35 | Vector size Ok. No promoter band visible. |
| flhDC promoter | 2926 | 2800 | 126 | Vector size Ok. No promoter band visible. Parcial digestion? |
| RBS+HlyD | 4296 | 2800 | 1496 | Fragment size Ok. Parcial digestion. |
| RBS-IL10 | 3345 | 2800 | 545 | Fragment size Ok. Parcial digestion. |
| RBS-IL12 | 4458 | 2800 | 1658 | Fragment size Ok. Parcial digestion. |
| RBS+HlyB | 4983 | 2800 | 2183 | Fragment size Ok. Parcial digestion, where´s linear vector? |
| RBS+HlyA | 3027 | 2800 | 227 | Fragment size Ok. Parcial digestion. |
| RBS+SoxR | 3323 | 2800 | 523 | Fragment size Ok. Parcial digestion. |
| RBS+QseB+RBS+QseC | 4886 | 2800 | 2086 | Fragment size Ok. Parcial digestion, where´s linear vector? |
- Repeat digestions with less DNA.
