Team:UNICAMP-EMSE Brazil/Notebook/21 September 2011
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Notebook
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Ligations:
=> Seq2 (~2200bp) and pSB1C3 (~2000bp) (ratio for ligation 1:1)
2.4 ul - H20
7.8 ul - digested Seq2 (~50 ng )
6.8 ul - digested vector [pSB] (~50 ng)
2 ul - 10X Ligation Buffer
1 ul - T4 Ligase
20 ul => TOTAL
=> Seq6 (~1200bp) and pSB1C3 (~2000bp) (ratio for ligation 2:1)
0.6 ul - H20
20 ul - digested Seq6 (~80 ng )
5.4 ul - digested vector [pSB] (~40 ng)
3 ul - 10X Ligation Buffer
1 ul - T4 Ligase
30 ul => TOTAL
=> Seq7 (5500bp) and pSB1C3 (~2000bp) (ratio for ligation 1:1)
3.3 ul - H20
6.9 ul - digested Seq7 (~50 ng )
6.8 ul - digested vector [pSB] (~50 ng)
2 ul - 10X Ligation Buffer
1 ul - T4 Ligase