Team:UNICAMP-EMSE Brazil/Notebook/5 September 2011
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05 September 2011
Checking digestion (E-P) of mini-preped constructs
Objective:
- Checking digestion (E-P) of mini-preped constructs. It was used larger amount of DNA for the digestion.
Samples:
- Gel 1:
- RBS+GFP+HlyA C1/ RBS+GFP+HlyA C2/ RBS+GFP+HlyA C3/ flhDC +RBS+IL12 C4/ SoxS+HlyA+RBS+IL10 C1/ SoxS+HlyA+RBS+IL10 C3/ SoxS+HlyA+RBS+IL10 C4/ SoxS+HlyB+HlyD+TolC+T C1/ flhDC+HlyB+HlyD+TolC+T C1
- Ladder:
- 100-10.000 bp (Fermentas)
- Gel Agarose concentration:
- 1,0%
- Results:
Gene | Total size (gene+vector) | Linear vector size | Gene size | Result |
---|---|---|---|---|
RBS+GFP+HlyA+T C1 | 4262 bp | 3205 bp | 1057 bp | No visible bands |
RBS+GFP+HlyA+T C2 | 4262 bp | 3205 bp | 1057bp | No visible bands |
RBS+GFP+HlyA+T C3 | 4262 bp | 3205 bp | 1057bp | No visible bands |
fLHDC+RBS+IL12 C4 | 4584 bp | 2800 bp | 1784 bp | OK |
SoxS+RBS+IL10+HlyA+T C1 | 4270 bp | 3205 bp | 1065 bp | No visible bands |
SoxS+RBS+IL10+HlyA+T C3 | 4270 bp | 3205 bp | 1065 bp | No visible bands |
SoxS+RBS+IL10+HlyA+T C4 | 4270 bp | 3205 bp | 1065 bp | No visible bands |
SoxS+RBS+HlyB+HlyD+TolC + T C1 | 8257 bp | 2800 bp | 5457 bp | Strange size |
fLHDC+RBS+HlyB+HlyD+TolC + T C1 | 8280 bp | 2800 bp | 5480 bp | OK |
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