Team:UNICAMP-EMSE Brazil/Notebook/5 September 2011

From 2011.igem.org

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Notebook

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May
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
June
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
July
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
August
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31


Electrophoresis Gel 1, 05/09/2011.
Gel 1: RBS+GFP+HlyA C1/ RBS+GFP+HlyA C2/ RBS+GFP+HlyA C3/ flhDC +RBS+IL12 C4/ SoxS+HlyA+RBS+IL10 C1/ SoxS+HlyA+RBS+IL10 C3/ SoxS+HlyA+RBS+IL10 C4/ SoxS+HlyB+HlyD+TolC+T C1/ flhDC+HlyB+HlyD+TolC+T C1

05 September 2011

Checking digestion (E-P) of mini-preped constructs

Objective:

  • Checking digestion (E-P) of mini-preped constructs. It was used larger amount of DNA for the digestion.

Samples:

  • Gel 1:
  • RBS+GFP+HlyA C1/ RBS+GFP+HlyA C2/ RBS+GFP+HlyA C3/ flhDC +RBS+IL12 C4/ SoxS+HlyA+RBS+IL10 C1/ SoxS+HlyA+RBS+IL10 C3/ SoxS+HlyA+RBS+IL10 C4/ SoxS+HlyB+HlyD+TolC+T C1/ flhDC+HlyB+HlyD+TolC+T C1
  • Ladder:
  • 100-10.000 bp (Fermentas)
  • Gel Agarose concentration:
  • 1,0%


  • Results:

Gene Total size (gene+vector) Linear vector size Gene size Result
RBS+GFP+HlyA+T C1 4262 bp 3205 bp 1057 bp No visible bands
RBS+GFP+HlyA+T C2 4262 bp 3205 bp 1057bp No visible bands
RBS+GFP+HlyA+T C3 4262 bp 3205 bp 1057bp No visible bands
fLHDC+RBS+IL12 C4 4584 bp 2800 bp 1784 bp OK
SoxS+RBS+IL10+HlyA+T C1 4270 bp 3205 bp 1065 bp No visible bands
SoxS+RBS+IL10+HlyA+T C3 4270 bp 3205 bp 1065 bp No visible bands
SoxS+RBS+IL10+HlyA+T C4 4270 bp 3205 bp 1065 bp No visible bands
SoxS+RBS+HlyB+HlyD+TolC + T C1 8257 bp 2800 bp 5457 bp Strange size
fLHDC+RBS+HlyB+HlyD+TolC + T C1 8280 bp 2800 bp 5480 bp OK




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