Team:UNICAMP-EMSE Brazil/Notebook/13 September 2011
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13 September 2011
Electrophoresis
Objective:
- Isolate and cut the inserts (volume of ladder applied 10uL; checking digestion sample: 40uL digested sample +4uL buffer (in two wells each time))
Samples:
- Gel 1:
- Const. promo+ RBS+ SoxR + Term (E/S, 2 samples); RBS+SoxR+T (X/P)
- Ladder:
- 100-10.000 bp (Fermentas)
- Gel Agarose concentration:
- 1,0%
- Results:
Construction | Total size (gene+vector) | Linear vector size | Gene size | Result |
---|---|---|---|---|
Const. promo+ RBS+ SoxR + Term (1) | ~ 3900 bp | ~ 3300 bp | 653 bp | Good digestion but the size seems too small. |
Const. promo+ RBS+ SoxR + Term (2) | ~ 3900 bp | ~ 3300 bp | 653 bp | Good digestion but the size seems too small. |
RBS+SoxR+T | ~3850 bp | ~ 3300 bp | 618 bp | Good digestion, size OK. |