Team:UNICAMP-EMSE Brazil/Notebook/14 September 2011

Electrophoresis Gel 1, 14/09/2011.
Gel 1: SoxS+RBS+IL-10 (E-S)

14 September 2011

Isolate and cut inserts

Objective:

• Isolate and cut the inserts (volume of ladder applied 6uL; checking digestion sample: 40uL digested sample +4uL buffer )

Samples:

• Gel 1:

• SoxS+RBS+IL-10 (E-S)

• Ladder:

• 100-10.000 bp (Fermentas)

• Gel Agarose concentration:

• 1,0%

• Results:

Preparing sequences to shipping

Objective:

• Preparing our sequences to insert into pSB1C3 and ship to MIT

Samples:

Electrophoresis Gel 2, 14/09/2011.
Gel 2 upper part: Ladder,flhDC C1,flhDC C2,flhDC C3, flhDC C4, SoxR C1, SoxR C2, SoxR C3, HlyA C1, HlyA C8, HlyA C9, HlyB(?) C1, HlyB(?) C2, HlyB(?) C3, HlyB(?) C7,
Bottom: Ladder, Qse C1, Qse C4, IL-12 C1, IL-12 C2

• Gel 2, upper part:

• Ladder | flhDC C1 | flhDC C2 | flhDC C3 | flhDC C4 | SoxR C1 | SoxR C2 | SoxR C3 | HlyA C1 | HlyA C8 | HlyA C9 | HlyB(?) C1 | HlyB(?) C2 | HlyB(?) C3 | HlyB(?) C7 |

• Gel2, bottom part:

• Ladder | Qse C1 | Qse C4 | IL-12 C1 | IL-12 C2

• Ladder:

• 100-10.000 bp (Fermentas)

• Gel Agarose concentration:

• 1,0%

• Results: