Team:UNICAMP-EMSE Brazil/Notebook/14 September 2011
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14 September 2011
Isolate and cut inserts
Objective:
- Isolate and cut the inserts (volume of ladder applied 6uL; checking digestion sample: 40uL digested sample +4uL buffer )
Samples:
- Gel 1:
- SoxS+RBS+IL-10 (E-S)
- Ladder:
- 100-10.000 bp (Fermentas)
- Gel Agarose concentration:
- 1,0%
- Results:
Construction | Total size (gene+vector) | Linear vector size | Gene size | Result |
---|---|---|---|---|
SoxS+RBS+IL-10 | 3448 bp | 2800 bp | 648 bp | Digestion and size OK |
Preparing sequences to shipping
Objective:
- Preparing our sequences to insert into pSB1C3 and ship to MIT
Samples:
- Gel 2, upper part:
- Ladder | flhDC C1 | flhDC C2 | flhDC C3 | flhDC C4 | SoxR C1 | SoxR C2 | SoxR C3 | HlyA C1 | HlyA C8 | HlyA C9 | HlyB(?) C1 | HlyB(?) C2 | HlyB(?) C3 | HlyB(?) C7 |
- Gel2, bottom part:
- Ladder | Qse C1 | Qse C4 | IL-12 C1 | IL-12 C2
- Ladder:
- 100-10.000 bp (Fermentas)
- Gel Agarose concentration:
- 1,0%
- Results:
Construction | Total size (gene+vector) | Linear vector size | Gene size | Result |
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