Team:UNICAMP-EMSE Brazil/Notebook/19 September 2011
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Ligations:
=> IL-10 (~550bp) and pSB1C3 (~2000bp) (ratio for ligation 3:1)
3.8 ul - H20
20 ul - digested IL-10 (~150 ng )
2.2 ul - digested vector [pSB] (~50 ng)
3 ul - 10X Ligation Buffer
1 ul - T4 Ligase
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30 ul => TOTAL
=> IL-12 (~1660bp) and pSB1C3 (~2000bp) (ratio for ligation 3:1)
3.9 ul - H20
15 ul - digested IL-12 (~180 ng )
2.6 ul - digested vector [pSB] (~60 ng)
2.5 ul - 10X Ligation Buffer
1 ul - T4 Ligase
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25 ul => TOTAL
=> HlyD (~1500bp) and pSB1C3 (~2000bp) (ratio for ligation 3:1)
3.9 ul - H20
15 ul - digested HlyD (~180 ng )
2.6 ul - digested vector (~60 ng)
2.5 ul - 10X Ligation Buffer
1 ul - T4 Ligase
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