Team:TU-Delft/Notebooks/Protocols/LBMedium
From 2011.igem.org
![TUDelft Logo2](http://upload.wikimedia.org/wikipedia/en/d/d6/IGEM_official_logo.png)
![TUDelft Logo2](http://www.osli.ca/uploads/images/base/logo.png)
![](http://www.dsm.com/en_US/static/common/img/banner-cworld-logo.png)
![TUDelft Logo2](http://home.tudelft.nl/fileadmin/UD/MenC/Support/Internet/TU_Website/TU_Delft_portal/Over_TU_Delft/Alumni/Verenigingen/Alumnivereniging/Alumnipas/Service/img/Logo_640_x_280.jpg)
![TUDelft Logo2](http://www.selectscience.net/images/logos/companies/1393_Greiner-Bio-One-Logo_Intern.jpg)
![TUDelft Logo2](http://edwingardner.com/projectivelandscape/site/img/UfD.gif)
![](https://static.igem.org/mediawiki/2011/2/20/Dia1.png)
![TUDelft Logo2](https://static.igem.org/mediawiki/2011/e/e0/TU_Delft_iGEM_logo.jpg)
-
Home
-
The Project What we are doing
-
The Team Who we are
-
Notebooks What we did
-
Human Practice Awareness
-
Safety Responsibilty
Protocol
LB Medium 1 liter
1. Measure 950 ml of demi water and put it in a flas
2. Add 10 grams of NaCl
3. Add 10 grams of Bacter Tryptone
4. Add 5 grams of Yeast extract
5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH
6. Fill up to 1 liter
7. Bring the flask to the autoclave
Retrieved from "http://2011.igem.org/Team:TU-Delft/Notebooks/Protocols/LBMedium"