Team:UEA-JIC Norwich/Methods

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University of East Anglia-JIC

High Efficiency Transformation Protocol

Culture Preparation Protocol

Glycerol Stock Solution Protocol

Qiagen Miniprep Protocol

Gel Electrophoresis Protocol

PCR protocol

PEG Transformation protocol

Algae Transformation using glass beads protocol

Algae Transformation using electroporation protocol

Starch Preparation protocol

1. Wash starch powder with 10mL distilled water and resuspend.

2. Centrifuge for five minutes at 4000rpm.

3. Pour off supernatant.

4. Resuspend with 500µL Ethanol.

5. Centrifuge for five minutes at 4000rpm.

6. Resuspend in 500µL TAP.

7. Centrifuge for five minutes at 4000rpm, pour off supernatant, and repeat once.

8. Resuspend in 500µL TAP with sucrose.

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