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User contributions
From 2011.igem.org
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- 21:41, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/polyatailing (top)
- 21:26, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/vectorcloning (top)
- 21:21, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/gelextract (Created page with "{{Banner}} 1. Excise the DNA fragment from the agarose gel with a clean, sharp scalpel. Minimize the size of the gel slice by removing extra agarose.<br> 2. Weigh the gel slice i...") (top)
- 21:18, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods (top)
- 21:13, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/sdm (top)
- 21:11, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/transformation (top)
- 21:09, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/pegtransformation (top)
- 21:08, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/transformation
- 21:06, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/miniprep (top)
- 20:45, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-moss (top)
- 20:27, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-algae
- 20:21, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-algae
- 20:21, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-algae
- 20:19, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-algae
- 20:14, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-moss
- 20:01, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices/Interviews (top)
- 18:35, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Attributions
- 18:33, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices/Intervieweight (top)
- 18:32, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices/Interviewsix (top)
- 18:32, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices/Interviewseven (top)
- 18:31, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices/Interviewsix
- 18:31, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices/Interviewfive (top)
- 18:31, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices/Interviewfive
- 18:03, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 16:42, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 15:55, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-algae
- 15:53, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-algae
- 15:52, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-algae
- 15:50, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Team
- 15:50, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 15:49, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices
- 15:48, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/ligation (Created page with "{{Banner}} 1. Add 2µl of 10× T4 DNA ligase buffer to an eppendorf tube.<br> 2. Add 50ng vector DNA to the mix.<br> 3. Add 50ng insert DNA to the mix.<br> 4. Add 20µl nuclease...") (top)
- 15:46, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/polyatailing (Created page with "{{Banner}} 1. Add 1.5µl purified PCR blunt-ended DNA fragments to a PCR tube.<br> 2. Add 10mM dATP at a final concentration of 0.2Mm to the tube.<br> 3. Add 0.6µl of 25mM MgCl...")
- 15:45, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/rdigest (Created page with "{{Banner}} 1. Add 0.5mg DNA to a sterile microcentrifuge tube.<br> 2. Add 2.5µl 10× NEBuffer to the tube.<br> 3. Add Nuclease free water to make the volume up to 25µl.<br> 4....") (top)
- 15:41, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 15:38, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/vectorcloning
- 15:32, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/vectorcloning (Created page with "{{Banner}} <html> 1. Centrifuge the pGEM-T easy vector and control insert DNA tubes to collect contents at the bottom of the tubes.<br> 2. Ligation reactions set up as described ...")
- 15:21, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 15:14, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/sdm
- 15:13, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/sdm
- 15:13, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/sdm
- 15:07, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/sdm
- 15:00, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/sdm (Created page with "{{Banner}} Site-directed mutagenesis (Control reaction) 1. 2. 3. 4. 5. 6. 7. 8. 9. 5µl of 10× reaction buffer. 5µl of pWhitescript control plasmid. 1.25µl (25ng) of contr...")
- 14:57, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:56, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:55, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/starchprep (top)
- 14:55, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/starchprep (Created page with "{{Banner}} <p style="color:#000000">1. Wash starch powder with 10mL distilled water and resuspend. <p style="color:#000000">2. Centrifuge for five minutes at 4000rpm. <p style="...")
- 14:54, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:53, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/algaeelectroporation (top)
- 14:51, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/algaeelectroporation (Created page with "{{Banner}} <p style="color:#000000">1. Transfer 50ml of algae culture into four 50ml screw top tubes. <p style="color:#000000">2. Place all four tubes in the eppendorf centrifug...")
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