Team:UEA-JIC Norwich/starchprep

From 2011.igem.org

University of East Anglia-JIC

UNIVERSITY OF EAST ANGLIA-JOHN INNES CENTRE

1. Wash starch powder with 10mL distilled water and resuspend.

2. Centrifuge for five minutes at 4000rpm.

3. Pour off supernatant.

4. Resuspend with 500µL Ethanol.

5. Centrifuge for five minutes at 4000rpm.

6. Resuspend in 500µL TAP.

7. Centrifuge for five minutes at 4000rpm, pour off supernatant, and repeat once.

8. Resuspend in 500µL TAP with sucrose.

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