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Team:UEA-JIC Norwich/rdigest
From 2011.igem.org
UNIVERSITY OF EAST ANGLIA-JOHN INNES CENTRE
1. Add 0.5mg DNA to a sterile microcentrifuge tube.
2. Add 2.5µl 10× NEBuffer to the tube.
3. Add Nuclease free water to make the volume up to 25µl.
4. Add 2.5µl of buffer B to the tube.
5. Add 5 units of the necessary restriction enzyme.
6. Gently mix the reaction by pipetting up and down and microcentrifuge briefly.
7. Incubate the contents at 37˚C for 60 minutes.
8. Perform heat inactivation by placing the tube in a 65˚C water bath for 15 minutes.
