Team:TU-Delft/Project/Modelling
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The intracellular module gives us information about the adhesiveness of Mfp-5 on the membrane which is directly dependent on the inducer (L-arabinose) concentration. It includes all steps in between the inducer (induction of the Mfp-5 expression?) and the amount of Mfp-5 eventually present on the outer membrane, such as for example the influence of the transcription factor, the efficiency of DNA-polymerase and the ribosome concentration in relation to the specific growth rate μ. | The intracellular module gives us information about the adhesiveness of Mfp-5 on the membrane which is directly dependent on the inducer (L-arabinose) concentration. It includes all steps in between the inducer (induction of the Mfp-5 expression?) and the amount of Mfp-5 eventually present on the outer membrane, such as for example the influence of the transcription factor, the efficiency of DNA-polymerase and the ribosome concentration in relation to the specific growth rate μ. | ||
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''Extracellular L-arabinose (Ara_out) is transported inside the cell (Ara_in). Here it binds to the transcription factor (TF) which becomes active (TF*) and binds to the promotor (P). The active promotor (P*) is transcribed by DNA-polymerase (pol) and the resulting mRNA is translated by ribosomes (ribo) into intracellular Mfp5-GFP-OmpA (Mfp5_in). This is transported by the Sec transport system to the periplasm. The periplasmic Mfp5-GFP-OmpA (Mfp5_p) folds and inserts itself into the outer membrane (Mfp5_m). When tyrosinase is present tyrosine groups will be hydroxylated to L-DOPA groups resulting in adhesive membrane-bound Mfp5-GFP-OmpA (Mfp5_m*). '' | ''Extracellular L-arabinose (Ara_out) is transported inside the cell (Ara_in). Here it binds to the transcription factor (TF) which becomes active (TF*) and binds to the promotor (P). The active promotor (P*) is transcribed by DNA-polymerase (pol) and the resulting mRNA is translated by ribosomes (ribo) into intracellular Mfp5-GFP-OmpA (Mfp5_in). This is transported by the Sec transport system to the periplasm. The periplasmic Mfp5-GFP-OmpA (Mfp5_p) folds and inserts itself into the outer membrane (Mfp5_m). When tyrosinase is present tyrosine groups will be hydroxylated to L-DOPA groups resulting in adhesive membrane-bound Mfp5-GFP-OmpA (Mfp5_m*). '' |
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