Team:UEA-JIC Norwich/starchprep

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Banner}} <p style="color:#000000">1. Wash starch powder with 10mL distilled water and resuspend. <p style="color:#000000">2. Centrifuge for five minutes at 4000rpm. <p style="...")
 
Line 1: Line 1:
{{Banner}}
{{Banner}}
-
<p style="color:#000000">1. Wash starch powder with 10mL distilled water and resuspend.
+
1. Wash starch powder with 10mL distilled water and resuspend.
-
<p style="color:#000000">2. Centrifuge for five minutes at 4000rpm.
+
 
-
<p style="color:#000000">3. Pour off supernatant.
+
2. Centrifuge for five minutes at 4000rpm.
-
<p style="color:#000000">4. Resuspend with 500µL Ethanol.
+
 
-
<p style="color:#000000">5. Centrifuge for five minutes at 4000rpm.
+
3. Pour off supernatant.
-
<p style="color:#000000">6. Resuspend in 500µL TAP.  
+
 
-
<p style="color:#000000">7. Centrifuge for five minutes at 4000rpm, pour off supernatant, and repeat once.
+
4. Resuspend with 500µL Ethanol.
-
<p style="color:#000000">8. Resuspend in 500µL TAP with sucrose.
+
 
 +
5. Centrifuge for five minutes at 4000rpm.
 +
 
 +
6. Resuspend in 500µL TAP.
 +
 
 +
7. Centrifuge for five minutes at 4000rpm, pour off supernatant, and repeat once.
 +
 
 +
8. Resuspend in 500µL TAP with sucrose.

Latest revision as of 14:55, 21 September 2011

University of East Anglia-JIC

UNIVERSITY OF EAST ANGLIA-JOHN INNES CENTRE

1. Wash starch powder with 10mL distilled water and resuspend.

2. Centrifuge for five minutes at 4000rpm.

3. Pour off supernatant.

4. Resuspend with 500µL Ethanol.

5. Centrifuge for five minutes at 4000rpm.

6. Resuspend in 500µL TAP.

7. Centrifuge for five minutes at 4000rpm, pour off supernatant, and repeat once.

8. Resuspend in 500µL TAP with sucrose.

Retrieved from "http://2011.igem.org/Team:UEA-JIC_Norwich/starchprep"