Team:UEA-JIC Norwich/Methods

From 2011.igem.org

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Revision as of 14:54, 21 September 2011

University of East Anglia-JIC

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 <a href="https://2011.igem.org/Team:UEA-JIC_Norwich/algaeelectroporation"> Algae Transformation using electroporation protocol </a>
-<p style="color:#000000">1. Transfer 50ml of algae culture into four 50ml screw top tubes.
+<br>
-<p style="color:#000000">2. Place all four tubes in the eppendorf centrifuge at 800rpm for 9 minutes at 20-22°C.
+<br>
-<p style="color:#000000">3. Immediately pour contents of tubes into glass beaker and keep tubes upside down so no unwanted suspension mixes with the pellet.
+<a href="https://2011.igem.org/Team:UEA-JIC_Norwich/starchprep"> Starch Preparation protocol </a>
-<p style="color:#000000">4. For all tubes resuspend cells in 250µl of TAP and sucrose media.
-<p style="color:#000000">5. Add linearised G-Luc plasmid (5µL)to the suspension and place on ice.
-<p style="color:#000000">6. Transfer 250µL of algae suspension into 1mL cuvette.
-<p style="color:#000000">7. Subject cuvettes to electroporation at 2200v/ cm and place back on ice.
-<p style="color:#000000">8. Add 500µL of starch solution to each cuvette and aspirate gently.
-<p style="color:#000000">9. Add 750µL of each sample onto an LB plate.
-<p style="color:#000000">10.Parafilm the lid and plate to seal and place in a light incubator.
-<h1 style="font-family:verdana;color:green">Starch Preparation</h1>
 <p style="color:#000000">1. Wash starch powder with 10mL distilled water and resuspend.
 <p style="color:#000000">2. Centrifuge for five minutes at 4000rpm.