Team:TU-Delft/Notebooks/Protocols/LBMedium
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==='''LB Medium 1 liter'''=== | ==='''LB Medium 1 liter'''=== | ||
- | 1. Measure 950 ml of demi water and put it in a | + | 1. Measure 950 ml of demi water and put it in a flas |
+ | |||
2. Add 10 grams of NaCl | 2. Add 10 grams of NaCl | ||
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3. Add 10 grams of Bacter Tryptone | 3. Add 10 grams of Bacter Tryptone | ||
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4. Add 5 grams of Yeast extract | 4. Add 5 grams of Yeast extract | ||
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5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH | 5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH | ||
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6. Fill up to 1 liter | 6. Fill up to 1 liter | ||
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7. Bring the flask to the autoclave | 7. Bring the flask to the autoclave | ||
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+ | [https://2011.igem.org/Team:TU-Delft/Notebooks/Protocols Back to Protocols...] | ||
+ | <br> | ||
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+ | {{TU-footer}} |
Latest revision as of 12:51, 9 August 2011