Team:TU-Delft/Notebooks/Protocols/PCR
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=='''Protocols''' == | =='''Protocols''' == | ||
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1. Make biobrick mastermix, containing per sample: | 1. Make biobrick mastermix, containing per sample: | ||
o 12.5 ul Taq mastermix | o 12.5 ul Taq mastermix | ||
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2. Put 25 ul in the PCR tubes. | 2. Put 25 ul in the PCR tubes. | ||
3. With a toothpick or pipet point, touch a colony and stir it through the fluid | 3. With a toothpick or pipet point, touch a colony and stir it through the fluid | ||
- | 4. Run the iGEM colpcr program (to be added later) | + | 4. Run the iGEM colpcr program (to be added later) |
=='''PCR using Taq Mastermix'''=== | =='''PCR using Taq Mastermix'''=== |
Revision as of 21:35, 21 September 2011