Team:TU-Delft/Notebooks/Protocols/PCR
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CarstenBlom (Talk | contribs) (→PCR) |
CarstenBlom (Talk | contribs) (→PCR using Taq Mastermix=) |
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4. Run the iGEM colpcr program (to be added later) | 4. Run the iGEM colpcr program (to be added later) | ||
- | =='''PCR using Taq Mastermix'''=== | + | ==='''PCR using Taq Mastermix'''=== |
Contents of the PCR mix is the for a large part the same as mentioned above for the Colony PCR. Differences will be noted here. First, instead of biobrick primer, any primer of choice can be added, also 2.5ul if standard solution has a concentration of 10 pmol/ul. Also x ul template DNA from a sample is added, where x depends on the total concentration of DNA in the sample. Typically 50 to 100 ng of total DNA is added. 7.5 - x ul of H2O is added to the mix. | Contents of the PCR mix is the for a large part the same as mentioned above for the Colony PCR. Differences will be noted here. First, instead of biobrick primer, any primer of choice can be added, also 2.5ul if standard solution has a concentration of 10 pmol/ul. Also x ul template DNA from a sample is added, where x depends on the total concentration of DNA in the sample. Typically 50 to 100 ng of total DNA is added. 7.5 - x ul of H2O is added to the mix. |
Revision as of 21:37, 21 September 2011