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Team:Queens Canada/Notebook/Protocols/GlycerolStock
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<h3red> Liquid Culture</h3red> | <h3red> Liquid Culture</h3red> | ||
| + | <regulartext> <b> Storage and Labelling </b> </regulartext> <br> | ||
| + | <regulartext> - Store the glycerol stock product in the -80°C freezer </regulartext> <br> | ||
| + | <regulartext> - Label the product tube as “EC (GL)” and in accordance with the standard labelling format, as outlined at the front of your lab book and on Google Docs</regulartext> <p> | ||
| + | <regulartext> <b> Materials </b> </regulartext> <br> | ||
| + | <regulartext> - Overnight bacterial cell culture </regulartext> <br> | ||
| + | <regulartext> - Cryogenic screw-cap vials </regulartext> <br> | ||
| + | <regulartext> - 30% glycerol in H2O</regulartext> <p> | ||
| + | <regulartext> <b> Procedure </b> </regulartext> <br> | ||
| + | <regulartext> 1. Pipette 750μL 30% glycerol into cryogenic vials. (Note: withdraw very slowly as glycerol is very viscous) <br> | ||
| + | 2. Add 750μL of overnight culture to each vial. <br> | ||
| + | 3. Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed. <br> | ||
| + | 4. Label each vial in accordance with labeling convention.<br> | ||
| + | 5. Store in a freeze box in -80⁰C freezer.<br> | ||
| + | <br> | ||
</div> | </div> | ||
Revision as of 13:00, 20 September 2011
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2. Add 750μL of overnight culture to each vial.
3. Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
4. Label each vial in accordance with labeling convention.
5. Store in a freeze box in -80⁰C freezer.
