Team:Queens Canada/Notebook/Protocols/Digestion

Storage and Labelling
- Use product immediately (if possible), or store in -20⁰C freezer
- Product should be labelled as "DNA (AQ)," using the standard labeling technique, as outlined in the front of your lab book and on Google Docs

Materials
- Digestion Buffer (10X, 2μL)
- Enzyme(s) (0.5-1μL)
- DNA (1μL)
- ddH2O (16-16.5μL)

Procedure
1. Remove the digestion buffer and enzymes from the -20⁰C freezer, place on ice.
2. Add 14 uL of water to an eppendorf tube.
3. Thaw the digestion buffer and the enzymes.
4. Add digestion buffer to tube.
5. Add DNA to tube.
6. Add enzymes to tube.
7. Incubate (time and temperature are variable and depend upon the enzymes used; incubate for 5-20min at 37⁰C)