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  • Team:Potsdam Bioware/Labjournal/August part 2
    <b>Time:</b> 2011-08-18, 15:00-18:00 <br> <b>Time:</b> 2011-08-19, 14:00-14:15<br>
    134 KB (19,391 words) - 13:03, 21 September 2011
  • Team:Potsdam Bioware/Labjournal/August part 1
    * Total volume: 15 µl * Total volume: 200 µl (approx. 15 µl per sequencing reaction)
    117 KB (17,234 words) - 01:03, 22 September 2011
  • Team:Potsdam Bioware/Labjournal/June
    <b>Time:</b> 2011-06-01,15:30-21:00<br> | 94 || 15 || Denaturation
    70 KB (9,678 words) - 01:31, 22 September 2011
  • Team:WHU-China/calendarjs
    ...."+a[12])*1E3:0);if(a[14]){b=Number(a[16])*60+(a[18]?Number(a[18]):0);b*=a[15]=="-"?1:-1;e=new Date(+e+b*60*1E3)}}return e}function mb(a){if(typeof a=="n ...a+"-widget-header",O=Wa+"-widget-content",x,A,ta,za,Da,Ea=i("slotMinutes")%15==0;x="<table style='width:100%' class='fc-agenda-days fc-border-separate' c
    72 KB (13,661 words) - 13:53, 24 October 2011
  • Script
    ...,41r-32,-26r31,-34r-42,-15r15,-35r41,19r-5,-45r44,0r-6,46r41,-20r15,35r-44,15","w":148},"^":{"d":"82,-252r52,0r77,143r-64,0r-39,-81r-40,81r-64,0","w":214
    34 KB (6,310 words) - 02:45, 7 September 2011
  • Team:WHU-China/Notebook/Daytoday
    <span style="font-weight:bolder">4/14~4/15</span>, preparing materials: such as DH5α, pDsRed -Express Vector, amp, ka <span style="font-weight:bolder">6/2~6/15</span>, produce the competent cell, use T4 ligase to join DNA fragments, an
    29 KB (3,834 words) - 14:34, 26 October 2011
  • Team:Potsdam Bioware/Labjournal/September part 2
    |Denaturation|| 94°C|| 15 s |DenaturationII || 94°C|| 15 s
    58 KB (8,180 words) - 00:26, 22 September 2011
  • Team:Potsdam Bioware/Labjournal/September part 1
    | 15 || pPDV089, clone 4 || 12|| ca. 1400 ** mycN: 15.2 ng/µl
    151 KB (21,501 words) - 17:06, 21 September 2011
  • Team:Potsdam Bioware/Labjournal/July
    <b>Time:</b> 2011-06-30,14:00-15:30<br> <b>Time:</b> 2011-06-24, 15:30-16:00 <br>
    55 KB (8,148 words) - 21:15, 18 September 2011
  • Team:TU Munich/lab/notebook/part3
    Annealing: 60°C, 15 s ...ng Zymo DNA Clean +Concentrator 25</p><p><i>ligation:</i></p><p>sample 1: 15µl PCR product 5µl vector
    121 KB (20,136 words) - 01:54, 22 September 2011
  • Team:Minnesota/Notebook
    | Thu, 07 Apr 2011 15:14:00 GMT | Mon, 18 Jul 2011 15:28:28 GMT
    32 KB (3,783 words) - 22:46, 27 September 2011
  • Team:Penn/project/protocols/
    • 15 mL conical tube<br /> <p>1. Warm media, PBS, trypsin in water bath to 37 C (Takes approximately 10-15 minutes)<br />
    86 KB (13,981 words) - 04:46, 29 September 2011
  • Team:Johns Hopkins/Notebook/Vio
    Followed same protocol as 6/24, trying not to pick too many cells, using .15µl of the 5µM primer mix, and extending the initial denaturation to 10 min =====7/15/2011=====
    78 KB (11,015 words) - 22:10, 28 October 2011
  • Team:Potsdam Bioware/Labjournal/October
    * centrifuge cell culture at 5000 x g/ 15 min * fill supernatant in a new 50 ml falcon and centrifuge again (5000 g/15 min)
    89 KB (13,149 words) - 18:03, 28 October 2011
  • Team:UT-Tokyo/Data/Method
    :#Centrifuge the incubative tube at 3,000g for 15 min with soft brake. :#Incubation at 16 &deg;C for 15-30 min.
    15 KB (2,260 words) - 23:25, 5 October 2011
  • Team:Michigan/Notebook
    ==Week 2 (5/15/2011-5/21/2011)== '''6/15/2011'''
    73 KB (11,794 words) - 05:23, 29 September 2011
  • Team:UT-Tokyo/LabNote
    ==='11/06/15(Wed)=== ==='11/07/15(Fri)===
    186 KB (30,010 words) - 20:25, 5 October 2011
  • Team:TU Munich/lab/notebook/part4
    </li><li>Ligation 15 </li><li>Ligation 15
    78 KB (13,436 words) - 22:42, 28 October 2011
  • Team:Debrecen Hungary/Protocols
    15, 50 ml tubes 1. Prepare the laminar flow box: turn on the ventillation, wait for 15 minutes, clean the bottom and the glass of the hood with 70% alcohol, and w
    51 KB (7,937 words) - 05:49, 22 September 2011
  • Team:Bielefeld-Germany/Backup-Protocols
    * Heat-shock cells for 15–20 seconds in a water bath at exactly 42 °C. Do not shake. ...Germany/Protocols#Used_enzymes | SAP (shrimp alcaline phosphatase)]] and 1.15 µL 10x SAP buffer to destination plasmid mix, continue incubation at 37 °
    62 KB (8,860 words) - 12:04, 15 September 2011
  • Team:TU Munich/lab/notebook/part1
    ... was performed as mentioned above and the cells were incubated over night (15 h) at 37 °C and cooled until monday at 4 °C.</p> ...: 15 ng; primer (5 µM)</p><p>6) DNA: 1,5 ng; primer (1 µM)</p><p>7) DNA: 15 ng; primer (1 µM)</p>
    49 KB (7,813 words) - 02:39, 22 September 2011
  • Team:Harvard/Protocols
    **annealing: 60-75C, 15 sec **extension: 72C, 15 sec (for under 1kb) or 30-60 sec per kb
    24 KB (4,085 words) - 03:27, 29 October 2011
  • Team:Freiburg/Modelling
    ...="color:black; background-color:lightgrey;" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" ...="color:black; background-color:lightgrey;" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" align=right
    21 KB (2,897 words) - 03:54, 22 September 2011
  • Team:OUC-China/Result/date
    ...">&micro;</span><span>l)</span></p></td><td class="c9"><p class="c4"><span>15</span></p></td><td class="c9"><p class="c4"><span>9</span></p></td></tr><tr
    146 KB (27,225 words) - 02:34, 29 October 2011
  • Team:Edinburgh/Lab Notebook
    At this point we also prepared about 15 plates of agar, with chloramphenicol (which the pSB1C3 vector provides resi '''June 15:'''
    69 KB (11,148 words) - 15:33, 3 October 2011
  • Team:Imperial College London/Sequence
    <p>53_AUX3 -- 15..793 of sequence</p> <p>53_premix -- 15..843 of sequence</p>
    41 KB (1,279 words) - 09:52, 20 September 2011
  • Team:MIT/Notebook/
    11:15 AM </td> <h3><a name="DailyNotebook-June15%2C2011"></a>June 15, 2011</h3>
    131 KB (18,449 words) - 03:35, 29 October 2011
  • Team:HokkaidoU Japan/WetLab
    |15 mM # Incubate the plate for 15-19 hrs at 37C
    14 KB (2,032 words) - 22:46, 3 October 2011
  • Team:Tokyo-NoKoGen/protocols
    2; Add 15 g of agar and stirrer bar.<br> 3; Centrifuge for 5 seconds at 15,000×g and 4 °C and discard supernatant.<br>
    17 KB (2,608 words) - 11:19, 5 October 2011
  • Team:METU-Ankara
    ... <li>Aliquot 10 ng of DNA in a volume 10 to 25  mL into a sterile 15 mL round bottom test tube and place on ice.</li> 15 uL 10X NE Buffer 2<br />
    134 KB (20,188 words) - 13:08, 11 October 2011
  • Team:NTNU Trondheim/Journal
    Incubated at room temperature for 30 minutes, then heated at 65 C for 15 minutes. == Friday 15/7 ==
    64 KB (10,273 words) - 15:51, 21 September 2011
  • Team:Macquarie Australia/Notebook
    :::Annealing – 52&deg;C for 30 seconds ] 15 cycles in total. ...s’ protocol with a few additions. Note that we made a mistake during our 15 cycle stage - it should be at 72&deg;C. Our bad. We have fixed this - the p
    27 KB (4,274 words) - 11:05, 4 October 2011
  • Team:METU Turkey SoftLab
    ... <li>Aliquot 10 ng of DNA in a volume 10 to 25  mL into a sterile 15 mL round bottom test tube and place on ice.</li> 15 uL 10X NE Buffer 2<br />
    134 KB (20,159 words) - 17:29, 13 October 2011
  • Team:HokkaidoU Japan/Protocols
    |15 mM # Incubate the plate for 15-19 hrs at 37C
    9 KB (1,356 words) - 13:34, 15 December 2011
  • Team:WashU/Notebook/June2011
    ****15 uL master mix ****15 uL master mix
    26 KB (4,070 words) - 16:08, 25 July 2011
  • Team:Uppsala-Sweden/Notebook
    ======2011-07-15====== ======2011-08-15======
    55 KB (8,809 words) - 15:16, 25 October 2011
  • Team:Freiburg/Description
    ...="color:black; background-color:lightgrey;" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" Width="20%" Align="right" ...="color:black; background-color:lightgrey;" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" align="right"
    23 KB (3,511 words) - 03:51, 22 September 2011
  • Team:WashU/Notebook/June
    ****15 uL master mix ****15 uL master mix
    30 KB (4,553 words) - 17:55, 19 July 2011
  • Team:Harvard/Template:NotebookData2
    **OZ052 (from 1:10 dilution): 15.2 ng/µL, 260/280=1.82 ...15.kan-ZFB-wp-his3-ura3(labeled).png|thumb|Kan and ZFB-wp-his constructs 6/15/11]]
    26 KB (3,991 words) - 23:44, 25 October 2011
  • Team:Valencia/Notebook/August
    | 13 || 14 || 15 || 16 || 17 || 18 || 19 || | 11 || 12 || 13 || 14 || 15 || 16 || 17 ||
    11 KB (1,565 words) - 17:12, 17 August 2011
  • Team:Gaston Day School/Notebook
    |15 |15
    6 KB (646 words) - 23:51, 27 September 2011
  • Team:Johns Hopkins/Notebook/YTExperiments
    =====07/15/11===== =====8/15/2011=====
    29 KB (4,167 words) - 03:47, 29 September 2011
  • Main page archive
    <img src="https://static.igem.org/mediawiki/igem.org/1/15/Forum.jpg" August 15, 2011
    70 KB (10,988 words) - 22:42, 7 November 2011
  • Team:BU Wellesley Software/Notebook/KyleNotebook
    6/15: Today I did the plasmid preps from the GFP+Term composite part and the K-r ...BS plasmid DNA for our constructs, I used the transformation plates from 6/15/11 to make liquid plasmid preps. The rest of the afternoon was spent plann
    50 KB (8,331 words) - 22:49, 28 September 2011
  • Team:Uppsala-Sweden/Project/Protocol
    * Add glycerol to 15% * Thaw the cells on ice for 15 minutes
    12 KB (1,960 words) - 23:24, 21 September 2011
  • Team:Harvard/Template:NotebookDataJuly4
    ***62˚ C, for 15 sec ... but the 10 ul plates only had a handful while the 50 ul plates had around 15-20 colonies each. We picked four colonies from each plate and grew them in
    29 KB (4,742 words) - 19:22, 3 August 2011
  • Team:UNIPV-Pavia/Measurements
    <li> 15 seconds of linear shaking (3 mm amplitude) followed by 5 seconds waiting be <li> duration time: 10 - 15 hours
    19 KB (3,055 words) - 00:40, 22 September 2011
  • Team:Harvard/Template:NotebookDataAugust
    This time it was incubated at 37*C for 15 minutes, and the product was PCR purified. **1-15 contained ECNR2+kan-ZFB-wp+zeocin+Zif268 spec plasmid
    26 KB (4,211 words) - 22:44, 19 September 2011
  • Team:Utah State/Notebook
    15. Remove the solution to just above the two plates in the first removal pipe 4. Add an additional 15 µL of WB buffer to the solution.
    24 KB (3,974 words) - 21:52, 28 September 2011
  • Team:Rutgers/LB1
    72 degrees C 15 sec<br /> <p>7/15<br />
    62 KB (8,401 words) - 02:39, 29 September 2011
  • Team:Hong Kong-CUHK/Notebook
    <div><b><u>15 June 2011 (Wed)</u></b></div> <div align="right" style="text-align:right">15</div>
    1.35 MB (148,320 words) - 17:48, 28 October 2011
  • Team:Hong Kong-CUHK/Laboratory/log book
    <div><b><u>15 June 2011 (Wed)</u></b></div> <div align="right" style="text-align:right">15</div>
    1.35 MB (148,301 words) - 15:24, 5 October 2011
  • Team:Copenhagen/Protocol
    0.1MCaCl/15%Glycerol (from a 85% stock) 2. Incubate for 15 mins on ice.
    20 KB (3,204 words) - 12:16, 26 August 2011
  • Team:Arizona State/Project/References
    ... bacterial chromosome,” J Biol Chem., vol. 280, no. 15, pp. 15111-15112, 15 April 2005.</div> ...Guide Antiviral Defense in Prokaryotes,” Science, vol. 321, pp. 960-964, 15 August 2008.</div>
    16 KB (2,107 words) - 02:44, 29 September 2011
  • Team:Amsterdam/Notebook/Calendar
    |width="15%" align="center" valign="top"|{{ #calendar: title=Team:Amsterdam |year=2011 |width="15%" align="center" valign="top"|{{ #calendar: title=Team:Amsterdam |year=2011
    733 B (81 words) - 10:35, 21 September 2011
  • Team:DTU-Denmark-2/Team/Protocols
    <td>15:00</td> ... buffer type 3-sample mix is mixed by inversion and incubated at 60°C for 15–30 minutes until the agarose is completely dissolved. The sample is mixed
    51 KB (7,868 words) - 09:05, 4 October 2011
  • Team:Harvard/Template:NotebookDataAugust3
    #Load 10 ul of protein ladder into gel, and load 15 ul of sample into each well. *EcNR2 strain with transformed zif268 plasmid grew on spec plates: After 15 hours, 1mL and 2mL plates have ~20 colonies each
    23 KB (3,770 words) - 22:30, 19 September 2011
  • Team:Bielefeld-Germany/Protocols/Genetics
    *Cool the culture 15-30 minutes on ice ...cultures into cooled 50 mL Falcons and centrifugate at 4000 rpm, 4 °C for 15 minutes, make sure to slowly accelerate and deccelerate
    23 KB (3,263 words) - 14:03, 13 October 2011
  • Team:Imperial College London/Project/Chemotaxis
    ...onsentrations of malate in milimolar range were tested, 0 mM, 5 mM, 10 mM, 15 mM, 20 mM, 25 mM. Circular colonies are observed with rising concentrations ...onsentrations of serine in milimolar range were tested, 0 mM, 5 mM, 10 mM, 15 mM, 20 mM, 25 mM. Circular colony can be observed at 0 mM concentration as
    44 KB (6,705 words) - 21:17, 14 September 2011
  • Team:Imperial College London/Project/Chemotaxis/Testing
    ...onsentrations of malate in milimolar range were tested, 0 mM, 5 mM, 10 mM, 15 mM, 20 mM, 25 mM. Circular colonies are observed with rising concentrations ...onsentrations of serine in milimolar range were tested, 0 mM, 5 mM, 10 mM, 15 mM, 20 mM, 25 mM. Circular colony can be observed at 0 mM concentration as
    22 KB (3,158 words) - 12:29, 14 September 2011
  • Team:Johns Hopkins/Notebook/YVL
    ...tary to each other with the base change in the exact middle of the primer. 15-20 bp of homology on either side of the base change is ideal. For a large b =====7/15/11=====
    9 KB (1,001 words) - 23:51, 28 October 2011
  • Workshops/Europe
    9:15. Talk: Synthetic Biology 10:15. Veteran team talk
    22 KB (3,045 words) - 20:23, 29 May 2011
  • Team:Lethbridge/Notebook/Lab Work/Group2
    ==Week 15 August 8 - 14== ==Week 16 August 15 - 21==
    13 KB (1,994 words) - 02:57, 29 September 2011
  • Team:Freiburg/Notebook
    | bgcolor="#FFFFFF" | [[Team:Freiburg/15_April|15]] | bgcolor="#CAE1FF" | [[Team:Freiburg/Notebook/15_May|15]]
    13 KB (1,543 words) - 02:08, 22 September 2011
  • Team:WashU/Notebook/July
    **15.0uL 10x buffer **EcoRI tube: 2 uL diluted DNA, 2 ul SH buffer, 1 uL EcoRI enzyme, 15 uL reaction
    6 KB (1,065 words) - 17:58, 19 July 2011
  • Team:BU Wellesley Software/Notebook/JanooNotebook
    --[[User:Jtferns|Jtferns]] 15:47, 7 June 2011 (CDT) <a class="al" href="#.5B6.2F15.2F2011.5D" title="">15</a> <span class="sep">|</span>
    48 KB (7,116 words) - 22:00, 27 September 2011
  • Team:Yale/Notebook/Week4
    <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week15">Week 15</a></li> <li>Centrifuged 4 100 mL solutions at 4700 rpm for 15 minutes at 4 degrees celsius, saved supernatant/pellet for 2 (frozen at -20
    7 KB (1,188 words) - 22:49, 27 September 2011
  • Team:Grinnell/Notebook/Protocols
    <li>Chill cells on ice for 15-120min (generally ~30min).</li> ...reYield Minicolumn and Collection Tube and centrifuge at maximum speed for 15 seconds. Discard the flow-through.</li>
    21 KB (3,695 words) - 04:12, 29 September 2011
  • Team:Harvard/Template:NotebookData
    *2) 94⁰C for 15 sec | C||0||0||15||0||0||3||0||0||0||5||0||0||6||0||31||6||14||0||0||0
    14 KB (1,886 words) - 14:52, 12 August 2011
  • Team:Minnesota/Protocols
    **15 g/L Agar (solid media only) **PEG 4000 15 g
    9 KB (1,369 words) - 03:54, 29 September 2011
  • Team:WashU/Notebook/July2011
    **15.0uL 10x buffer **EcoRI tube: 2 uL diluted DNA, 2 ul SH buffer, 1 uL EcoRI enzyme, 15 uL reaction
    18 KB (2,731 words) - 04:45, 28 September 2011
  • Team:Cornell/Protocol
    :::#Inactivate CIAP by heating at 85°C for 15 minutes :::#Wait 15 minutes
    17 KB (2,930 words) - 03:58, 29 September 2011
  • Team:Harvard/Template:NotebookDataJuly2
    *15.0 ul isothermal assembly solution We had an annealing temperature of 58* and an extension time of 15 seconds.
    33 KB (5,222 words) - 18:48, 3 August 2011
  • Team:Imperial College London/Project/Chemotaxis/Results
    ...onsentrations of malate in milimolar range were tested, 0 mM, 5 mM, 10 mM, 15 mM, 20 mM, 25 mM. Circular colonies are observed with rising concentrations ...onsentrations of serine in milimolar range were tested, 0 mM, 5 mM, 10 mM, 15 mM, 20 mM, 25 mM. Circular colony can be observed at 0 mM concentration as
    39 KB (5,767 words) - 09:46, 8 September 2011
  • Team:Nevada/Notebook/Weeks912
    LT:Four colonies (1,4,9,& 15) from the initial 15 were selected and inoculated in LB-Amp broth. Cultures were purified using Colonies #1,4,9, & 15 were re-cultured in LB-Amp broth, purified using the QIAquick miniprep kit,
    30 KB (5,035 words) - 03:20, 29 September 2011
  • Team:Arizona State/Templates/cas table
    ! Name {{:Team:Arizona State/Templates/ref|15}} ...|01587}} || || || Helicase, nuclease {{:Team:Arizona State/Templates/ref|15}} ||
    12 KB (995 words) - 01:59, 29 September 2011
  • Summer events/Turkish Team Meetup
    10:45-11:15 Tea Break 11:15-12:30 Presentations of iGEM Teams
    3 KB (419 words) - 12:38, 9 September 2011
  • Team:Fatih Turkey/Procedures
    - 30 mL glycerol (15% v/v)<br /> - 15 mL glycerol (15% v/v)<br />
    18 KB (2,277 words) - 19:51, 28 October 2011
  • Team:Bielefeld-Germany/Protocols/Downstream-processing
    * reaction mixture was centrifuged for 30 - 90 min at 15,000 ''g'' at 4 °C * Centrifuge the cell suspension for 15 min at 14,000 g (4 °C).
    23 KB (3,137 words) - 01:53, 29 October 2011
  • Team:Nevada/Notebook/Weeks1316
    ...HIFI and PstI, along with GLF (5) in pUC57 and pSBIC3. Colonies 9, 11, and 15 on the gel looked correct, showing two bands at 2000bp and 1600bp. They wil LT:Colonies #1-15 were selected from the BTE/trc promoter transformation, inoculated in TB-CH
    25 KB (4,235 words) - 03:58, 29 September 2011
  • Team:Imperial College London/Project Chemotaxis Testing
    <td><p>15 mM</p></td> ...ircular colony, b)5 mM - elliptical colony c) 10 mM - elliptical colony d) 15 mM - elliptical colony e) 20 mM - elliptical colony f) 25mM - elliptical co
    26 KB (4,242 words) - 03:45, 29 October 2011
  • Team:Bilkent UNAM Turkey/Team Meetup
    <li>10:45-11:15 Coffee Break <li>11:15-12:30 Presentations of iGEM Teams teams
    7 KB (974 words) - 04:50, 22 September 2011
  • Team:DTU-Denmark-2/Project/background
    ... in 1964. The original cells were taken from bone tissue of the tibia of a 15 year old girl suffering from osteosarcoma. An immortalized cell line has ac ...te linear DNA fragments in its genome by repair of double stranded breaks (15). </p>
    15 KB (2,211 words) - 08:40, 21 September 2011
  • Team:EPF-Lausanne/Our Project/TetR mutants/MITOMI data
    | 15||0.540091||0.8821||1.0861||0.164072 | 15||0.540091||0.8821||1.0861||0.164072
    16 KB (1,939 words) - 21:49, 26 October 2011
  • Team:ULB-Brussels/mat
    <li>Incubate 15 minutes at 42°C</li> <li>Incubate 15 minutes at 70°C.</li>
    29 KB (4,276 words) - 02:03, 22 September 2011
  • Team:NCTU Formosa/calendar
    ...oduct<br/><td ></tr><tr><td class='s4'>9/16/2011<td class='s12'>1. PCR 9/15 transform product &amp;check by electrophoresis 2. PCR vioABE+1C3// night
    46 KB (7,856 words) - 13:40, 5 October 2011
  • Team:UNAM-Genomics Mexico/Notebook/nifH
    ==Week: July 11- July 15== ==Week: August 15 - August 19==
    13 KB (2,129 words) - 21:37, 28 September 2011
  • Team:UNAM-Genomics Mexico/Notebook/repC
    ==Week: August 15 - August 21== <td>10μL Gibson HydG 15/Sep&nbsp;<em>Pae</em></td>
    8 KB (1,256 words) - 19:48, 28 September 2011
  • Team:Fatih Turkey/ecolicompetent
    - 30 mL glycerol (15% v/v)<br /> - 15 mL glycerol (15% v/v)<br />
    18 KB (2,277 words) - 19:52, 28 October 2011
  • Team:Johns Hopkins/mootoolsjs
    ...1Q\',k(){B(c.4X)c.G.2K().28(c.3c)})},4q:k(){M(o i=0;i<2;i++)c.12[i]=c.4o(c.15[i],c.17[i])},8Q:k(){c.34=\'34-1o\';c.64=\'2N\';c.1E=c.G.3R},8M:k(){c.34=\'3
    43 KB (12,115 words) - 05:15, 28 October 2011
  • Team:Lethbridge/Notebook/Lab Work/Group1
    ==June 15-16, 2011== *The SDS page was run at 80Volts for 15 mins and 180Volts for 45mins
    22 KB (3,366 words) - 03:32, 29 September 2011
  • Team:BU Wellesley Software/Notebook/EvelynNotebook
    Week 7/4-7/15 Week 7/15-8/1
    29 KB (4,664 words) - 00:38, 29 September 2011
  • Frank Machin's Lab Notebook
    ..."11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"}, ...e_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
    25 KB (3,664 words) - 21:23, 3 July 2011
  • Team:Paris Bettencourt/Notebook
    ...https://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/06/15/">15</a></td> ...https://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/07/15/">15</a></td>
    19 KB (2,638 words) - 09:33, 21 October 2011
  • Team:UNIPV-Pavia/Calendar/June/settimana5
    <p>11 LB agar plates, 35 LB agar + Amp plates and 15 LB agar + Cm 12.5 were prepared.</p> ...0</A> was resuspended from iGEM 2011 kit distribution, Plate 1, well 4K in 15 &mu;l of ddH<small><sub>2</sub></small>O
    5 KB (867 words) - 17:34, 17 August 2011
  • Atipat Patharagulpong's Lab Notebook
    ..."11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"}, ...e_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
    26 KB (3,696 words) - 20:45, 3 July 2011
  • Christopher Schoene's Lab Notebook
    ..."11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"}, ...e_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
    24 KB (3,555 words) - 10:05, 4 July 2011
  • Nicolas Kral's Lab Notebook
    ..."11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"}, ...e_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
    25 KB (3,696 words) - 10:08, 4 July 2011
  • Team:Imperial College London/Extras/Brainstorming
    - Prodigiosin biosynthesis gene cluster (pig cluster) contains ~15 ORFs in <EM>Serratia</EM> strains (Harris <EM>et al.,</EM> 2004)<br> venoms are produced by snakes where 15% of 3,000 species of snakes are found poisonous. Snake
    67 KB (10,740 words) - 10:07, 22 July 2011
  • Team:BYU Provo/Protocols
    ...n, and set power supply to between 130 and 170 volts. It will take between 15 and 30 minutes to run, depending on the desired resolution. ==Ligation (15 μl reaction)==
    11 KB (1,803 words) - 22:50, 28 October 2011
  • Team:Harvard/Notebook
    if(k==15){ 15
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  • Team:Peking S/lab/notebook/shd
    |style="text-align:center"| 15 |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#7.8 - 7.15|8]]
    19 KB (2,592 words) - 05:05, 5 October 2011
  • Team:Harvard/Template:NotebookDataJuly3
    ...(labeled).png||thumb|none|Different attempts and thio kan-ZFB-hisura PCR 7/15/11]] 5 84_1 15 83_3
    32 KB (5,125 words) - 20:53, 4 August 2011
  • Regions/Americas/Pre-Jamboree
    * [[#15|Submit your Judging Form]] ...ink=1 this page] for more information. The submission due date was '''July 15, 2011'''.
    22 KB (3,075 words) - 01:15, 29 September 2011
  • Team:Tec-Monterrey/projectresults
    width: 15%; ...8)SF - TNI (9)IF (10) SF - WT (11)IF (12)SF.(13) PMWM. BL21SI, TI (14)IF (15)SF. TI – transformed and induced. TNI – transformed and no induced, WT
    24 KB (3,144 words) - 21:16, 20 October 2011
  • Team:EPF-Lausanne/Notebook/August2011
    == Monday, 15 August 2011 == 15. T7 lac2 111
    39 KB (6,395 words) - 09:04, 21 September 2011
  • Team:Osaka/Notebook
    |<span style={{{s15|"color:{{{c15|red}}};"}}}>15</span> |[[Team:Osaka/week7#September 15 (Thu)|15]]<span style={{{s15|"color:{{{c15|inherit}}};"}}}></span>
    12 KB (1,674 words) - 10:32, 28 October 2011
  • Team:KAIST-Korea/Notebook/Week2
    ...e="width:100%; text-align:center;" cellspacing="0" border="0" cellpadding="15" |[[Team:KAIST-Korea/Notebook/Week15|Week 15]]
    8 KB (1,254 words) - 13:12, 20 August 2011
  • Team:UNIPV-Pavia/Calendar/June/week5
    <p>11 LB agar plates, 35 LB agar + Amp plates and 15 LB agar + Cm 12.5 were prepared.</p> ...0</A> was resuspended from iGEM 2011 kit distribution, Plate 1, well 4K in 15 &mu;l of ddH<small><sub>2</sub></small>O
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  • Team:ITESM Mexico/Protocols
    ::#When the O.D. 600=0.35 put the cells on new tubes on ice for 10-15 mins (keep cold form now on). ::#Incubate on ice for 15 min
    24 KB (3,666 words) - 03:16, 29 September 2011
  • Team:KULeuven/Protocols
    <li>Streak the TOP10F<sup>1</sup> cells on a tetracyclin (15 <i>μg/ml</i>) streptomycine (20 <i>μg/ml</i>) LB-agar plate and grow over ... store at -70 &deg;C with addition of glycerol to a final concentration of 15%</li>
    16 KB (2,769 words) - 17:06, 21 September 2011
  • Team:UTP-Panama/Week 15
    ==Week 15: September 12 to 17== 15. Added to each tube 1000µL of PCA (Phenol:Chloroform:Isoamyl alcohol)<br>
    3 KB (454 words) - 03:50, 29 September 2011
  • Team:EPF-Lausanne/Notebook/September2011
    ...are: T7 030 (3), T7 054 (4), T7 111 (6), T7 lac2 054 (13) and T7 lac2 111 (15). We will focus on them and try to build biobrick formats before the deadli ...biobrick variants and made colony PCRs on five colonies of variats 3,4 and 15. The resuklts show that we have 2 good colonies for 3 and one for 5.
    15 KB (2,527 words) - 08:43, 21 September 2011
  • Team:UANL Mty-Mexico/Project/Mechanism
    ...re taken and modified from Dodd BI <i>et al</i> (2001) <i>Gene Dev</i>. <b>15</b>:3013–3022. "> ...re taken and modified from Dodd BI <i>et al</i> (2001) <i>Gene Dev</i>. <b>15</b>:3013–3022. </span></div>
    22 KB (3,420 words) - 16:53, 13 February 2012
  • Team:Imperial College London/Project Auxin Testing
    ...is corresponded to IAA and looked for the same peak in our samples between 15 and 16 minutes. </p> <p><i> Figure 15: Plot of root length data showing the effect of IAA concentration on root g
    57 KB (9,190 words) - 03:19, 29 October 2011
  • Team:Johns Hopkins/Notebook/Protocols
    ... change (single point mutation vs large deletion/insertion) I usually have 15-40nt on either side with perfect match. Order primer and its reverse comple | 15 *
    11 KB (1,770 words) - 23:26, 28 September 2011
  • Team:Groningen/project notebook/27 July 2011
    ...et. Gently resuspend each pellet. Leave the resuspended pellets on ice for 15 minutes. <br>10. Get some liquid nitrogen. <br>11. Dispense 4 ml of resuspe
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  • Team:Groningen/cloning
    ...ollect culture into 8 x 50ml pre-cooled centrifuge tubes. Leave on ice for 15 minutes. <br>4. Pellet the cells by centrifuging at 2700 rpm for 15 minutes 4 °C.
    8 KB (1,363 words) - 22:07, 21 September 2011
  • Team:DTU-Denmark-2/results/background
    ...te linear DNA fragments in its genome by repair of double stranded breaks (15). </p> ...ppear to favour NHEJ over HR resulting in low gene targeting efficiencies (15). </p>
    15 KB (2,208 words) - 14:44, 21 September 2011
  • Team:Groningen/project notebook/17 June 2011
    ...ollect culture into 8 x 50ml pre-cooled centrifuge tubes. Leave on ice for 15 minutes. <br>4. Pellet the cells by centrifuging at 2700 rpm for 15 minutes 4 °C.
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  • Team:Calgary/Notebook/Calendar/Binf
    <h3> July 11-15, 2011</h3> ...g |thumb|500px|center|<p><b>Figure 6.</b> 0.8% Agarose Gel run at 110V for 15 minutes for RNA preps (not DNAse treated) from Pseudomonas LD2 treated with
    26 KB (4,353 words) - 03:58, 29 October 2011
  • Team:Brown-Stanford/Lab/Notebook/Week15
    *-> 15 ng/μl (~50%) ...100 mL water, pH to 6.8 ± .2, filter sterilize. Autoclave 900 mL water w/ 15 g agar. Combine and plate.
    4 KB (614 words) - 18:10, 28 September 2011
  • Team:Arizona State/Notebook/August
    == Monday, August 15 == :* Keith's 2x RA and Seq1 streak plates from 8/15 (grew this time!)
    19 KB (2,982 words) - 03:06, 29 September 2011
  • Team:Grinnell CN/Notebook/Protocols
    <li>Chill cells on ice for 15-120min (generally ~30min).</li> ...reYield Minicolumn and Collection Tube and centrifuge at maximum speed for 15 seconds. Discard the flow-through.</li>
    19 KB (3,336 words) - 04:14, 27 September 2011
  • Team:Peking S/lab/notebook/wdq
    |style="text-align:center"| 15 |style="text-align:center"| [[Team:Peking_S/lab/notebook/wdq#7.8 - 7.15|8]]
    18 KB (2,417 words) - 05:07, 5 October 2011
  • Team:Hong Kong-CUHK/Laboratory log book
    <strong><u>15 June 2011 (Wed)</u></strong> 15
    278 KB (35,620 words) - 04:07, 3 October 2011
  • Team:Peking S/lab/notebook/jwy
    |style="text-align:center"| 15 |style="text-align:center"| [[Team:Peking_S/lab/notebook/wdq#7.8 - 7.15|8]]
    18 KB (2,417 words) - 05:21, 5 October 2011
  • Team:Greenfield IN-Schini-HS/Notebook
    <TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">15</font><br><br><br><br></TD> <TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">15</font><br><br><br><br></TD>
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  • Regions/Asia/Jamboree/Pre-regional Jamboree
    <li><a href="#15">Submit your Judging Form</a></li> ...nk=1"> page </a> for more information. The submission due date was <strong>15 July</strong>.</p>
    27 KB (4,381 words) - 12:45, 13 October 2011
  • Team:Bielefeld-Germany/Labjournal
    ...ny-2011 TAMRA-emissionspectra-544nm target.png|450px|thumb|left| '''Figure 15: Emission spectra of TAMRA labeled molecular beacon in its closed or open s ==Week 15: 8th August - 14th August==
    32 KB (4,418 words) - 23:09, 28 October 2011
  • Team:Imperial College London/Brainstorming Health
    ...cer cells) - Prodigiosin biosynthesis gene cluster (pig cluster) contains ~15 ORFs in Serratia strains (Harris et al., 2004) - PigS and PigP regulate pro ...pes of animals. One of the most common venoms are produced by snakes where 15% of 3,000 species of snakes are found poisonous. Snake venom consists of pr
    29 KB (4,673 words) - 14:57, 21 September 2011
  • Team:Brown-Stanford/Lab/Notebook
    [[Team:Brown-Stanford/Lab/Notebook/Week1|15]] [[Team:Brown-Stanford/Lab/Notebook/Week5|15]]
    12 KB (1,200 words) - 18:05, 28 September 2011
  • Team:BU Wellesley Software/Notebook/AlbertoNotebook
    ...t-family: helvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h3>8/15/2011-8/21/2011</h3> </td><td>Work for 10-15 hours a week
    51 KB (8,151 words) - 05:01, 29 September 2011
  • Team:Brown-Stanford/lightbox/js
    ...av-btnPrev').css({ 'background' : 'url(' + settings.imageBtnPrev + ') left 15% no-repeat' }) $(this).css({ 'background' : 'url(' + settings.imageBtnPrev + ') left 15% no-repeat' });
    19 KB (2,135 words) - 01:29, 12 June 2011
  • Team:EPF-Lausanne/Protocols/TetR
    :<b>4.</b> 72°C for 15 sec; you have to calculate the time required. This polymerase has an effciency of 15-30s/kb.(calculate for the longest fragment)
    3 KB (452 words) - 08:48, 15 July 2011
  • Team:BU Wellesley Software/Notebook/MargauxNotebook
    ...tica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h5>Week of 7/8 to 7/15</h5> ...: helvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h5>Week of 7/15 to 7/22</h5>
    30 KB (4,744 words) - 23:34, 27 September 2011
  • Team:BU Wellesley Software/Notebook/JanooNotebook/test
    <td>15</td> <td>15</td>
    3 KB (529 words) - 04:12, 27 June 2011
  • Team:Colombia/Notebook
    ===July 15=== * Digestions for 3 hours at 37C and inactivated at 65C for 15 seconds.
    10 KB (1,441 words) - 18:10, 27 September 2011
  • RecA: Week 5, June 13-18
    ==Wednesday, June 15== &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;DNA from the cultures created on 6/15 containing the terminator (B0015) and the PcI reporter (I763007) was extrac
    5 KB (754 words) - 03:01, 29 September 2011
  • Reporter: Week 5 June 12-17
    In this reaction, 15.5 &mu;l of dH<sub>2</sub>O and 0.5 &mu;l of MgSO<sub>4</sub> were added to ==Wednesday, June 15==
    10 KB (1,638 words) - 17:26, 13 July 2011
  • Team:Glasgow/Modeling
    ...low, 1.25x10<sup>-17</sup> [μmol/cell], the Vmax of PDE is 1.5457x10<sup>-15</sup> [μmol min<sup>-1</sup> molecule<sup>-1</sup>]. This means an average ...ws cells illuminated for 29 min and the second shows cells illuminated for 15 min. Plots show concentration of the protein after 360 min of diffusion. Th
    18 KB (2,819 words) - 15:18, 20 September 2011
  • Team:EPF-Lausanne/Notebook/July2011
    pSB3K1, colony 1: 15.5 ng/microL ...bone with Ptet (from newly assembled J61002 plasmid), final concentration: 15.1 ng/ul
    33 KB (5,283 words) - 16:18, 2 August 2011
  • Team:Arizona State/Notebook/July
    ::* ((used 22.5 uL water, 15 uL insert, 5 uL vector, 5 uL T4 buffer, 2.5 uL T4 ligase)) == Friday, July 15 ==
    23 KB (3,494 words) - 02:54, 29 September 2011
  • Team:Arizona State/Project/CRISPR
    ...casE, aka cse1, cse2, cse3, cse4, cas5e{{:Team:Arizona State/Templates/ref|15}}. These 5 proteins combine to form the Cascade complex{{:Team:Arizona Stat ...nisms and here referred to as Cas1-Cas6{{:Team:Arizona State/Templates/ref|15}}.</p>
    16 KB (2,419 words) - 02:53, 29 September 2011
  • Team:BU Wellesley Software/Notebook/ShannonNotebook
    | 15.4 ...vetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h5>WEEK 6: 7/11-7/15/2011</h5>
    20 KB (2,751 words) - 01:27, 29 September 2011
  • Team:Cornell/Week 15
    ==Thursday, September 15== :*Desalted the light-lysis + pSB1C3 ligation and control on a membrane for 15 minutes.
    6 KB (976 words) - 17:24, 18 September 2011
  • Team:Peking R/Notebook/LZ
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,432 words) - 13:44, 16 September 2011
  • Team:Washington/scripts/Lightbox
    ...av-btnPrev').css({ 'background' : 'url(' + settings.imageBtnPrev + ') left 15% no-repeat' }) $(this).css({ 'background' : 'url(' + settings.imageBtnPrev + ') left 15% no-repeat' });
    19 KB (2,131 words) - 22:29, 20 July 2011
  • Team:Peking S/lab/notebook/yrf
    |style="text-align:center"| [[Team:Peking_S/lab/notebook/yrf#7.15|15]] ===7.15===
    11 KB (1,328 words) - 12:03, 19 August 2011
  • Team:Wisconsin-Madison/protocols
    </li><li> Repeat steps 14 and 15 to remove all traces of isopropanol. Pulse spin after removing bulk of fina </li><li> Air dry the pellet for ~15 minutes (pellet will change from white to clear as it dries). Resuspend in
    27 KB (4,151 words) - 23:09, 28 September 2011
  • Team:USTC-Software/meetup
    </tr><tr><td>14:10-15:25</td> </tr><tr><td>15:25-15:35</td>
    7 KB (1,061 words) - 06:51, 23 October 2011
  • Team:Peking S/lab/notebook/sr
    |style="text-align:center"| [[Team:Peking_S/lab/notebook/yrf#7.15|15]] ===7.15===
    13 KB (1,604 words) - 05:20, 5 October 2011
  • Team:Osaka/week5
    #* 15(C):2-16N(upstream)+1-23L(downstream)+1-3A(vector) # Transfer to liquid culture:12,13,14,15,16
    2 KB (254 words) - 05:52, 19 October 2011
  • Team:UNICAMP-EMSE Brazil/protocols/chimiocompetent prepare
    |15%(m/V) |0.15 g
    7 KB (1,090 words) - 20:27, 27 September 2011
  • Team:UTP-Panama/Week 11
    ==Week 11: August 15 to 20== == August 15==
    4 KB (678 words) - 03:59, 29 September 2011
  • Team:WITS-CSIR SA/Project/Characterization
    ...the emission peak was detected at 528 nm. Twenty readings were taken every 15 minutes for each culture, for a total period of 135 minutes. An empty vecto ...607 nm using a Jasco FP-6300 spectrofluorometer. Readings were taken every 15 minutes for 120 minutes in total. The same empty vector was used as a negat
    36 KB (5,022 words) - 17:19, 6 October 2011
  • Team:Bilkent UNAM Turkey/TEST
    if(k==15){ 15
    14 KB (1,904 words) - 05:45, 4 September 2011
  • Labjournal may
    <b>Time:</b> 2011-05-12, 4:00 pm - 8:00 pm and 2011-05-13, 11:15 am - 12:30 am <br> <b>Time:</b> 2011-05-26,17:45-18:15<br>
    17 KB (2,404 words) - 15:34, 5 September 2011
  • Team:Northwestern/lightbox/js
    ...av-btnPrev').css({ 'background' : 'url(' + settings.imageBtnPrev + ') left 15% no-repeat' }) $(this).css({ 'background' : 'url(' + settings.imageBtnPrev + ') left 15% no-repeat' });
    19 KB (2,130 words) - 18:05, 24 September 2011
  • Team:TU Munich/model/combineddata
    ...1998/Math/MathML'> <mrow> <msub> <mrow><mi>x</mi> </mrow> <mrow><mn>15</mn> </mrow> </msub> </mrow></math></div></td><td align='center' valign=
    100 KB (14,899 words) - 11:42, 28 October 2011
  • Team:Lethbridge/Notebook/Lab Work/Justin
    =Week 2 (May 9 - 15)= <p>A 15% SDS-PAGE of samples taken from the BamH1 over-expression was ran. Gel look
    18 KB (2,706 words) - 03:50, 29 September 2011
  • Team:Potsdam Bioware/Meetings
    ==Protokoll zum Treffen am 15.9.2011== ==Protokoll zum Treffen am 15.08.2011==
    74 KB (9,960 words) - 01:06, 22 September 2011
  • Team:Potsdam Bioware/Labjournal/May
    <b>Time:</b> 2011-05-12, 4:00 pm - 8:00 pm and 2011-05-13, 11:15 am - 12:30 am <br> <b>Time:</b> 2011-05-26,17:45-18:15<br>
    16 KB (2,275 words) - 17:57, 21 September 2011
  • Team:Kyoto/Hunger
    ...ded 2.4ml media(-casamino acids) at 37 °C. We brought up E.coli at 0,5,10,15,20,25,30,60min and mesured OD600 and extracted RNA and synthesized cDNA. Fi ... for the internal control and that E.coli is not in the steady state after 15 minutes in this experiment.
    15 KB (2,175 words) - 03:56, 6 October 2011
  • Team:Tec-Monterrey/projectprotocols
    width: 15%; 15.Remove the liquid from the collection tube and replace the minicolumn.
    30 KB (4,184 words) - 20:53, 20 October 2011
  • Team:METU-Ankara/lab
    ... <li>Aliquot 10 ng of DNA in a volume 10 to 25  mL into a sterile 15 mL round bottom test tube and place on ice.</li> 15 uL 10* NE Buffer 2<br />
    21 KB (3,185 words) - 10:54, 21 September 2011
  • Team:BYU Provo/Team OxyR/Week9
    ==June 15== Re-did colony PCR from June 15.
    617 B (78 words) - 23:30, 27 September 2011
  • Team:Johns Hopkins/Notebook/DNAAssayProtocol
    ...5 ml for a 500 ml culture) of ice '''cold 0.1M CaCl<sub>2</sub> containing 15% glycerol'''. You can let the cells sit on ice here for a while as well. # Heat shock the preparations at 42<sup>o</sup>C for 15 minutes.
    18 KB (2,755 words) - 23:30, 28 September 2011
  • Team:Peking R/Notebook/ZYY/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,437 words) - 17:24, 25 September 2011
  • Team:Peking R/Notebook/CSB/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,449 words) - 17:26, 25 September 2011
  • Team:Peking R/Notebook/LZ/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,438 words) - 17:20, 25 September 2011
  • Team:Peking R/Notebook/GY/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,442 words) - 17:30, 25 September 2011
  • Team:Peking R/Notebook/XQY/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,455 words) - 17:27, 25 September 2011
  • Team:Peking R/Notebook/ZZR/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,479 words) - 17:29, 25 September 2011
  • Team:Peking R/Notebook/LYP/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,432 words) - 17:21, 25 September 2011
  • Team:Peking R/Notebook/HSY/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,461 words) - 17:23, 25 September 2011
  • Team:Peking R/Notebook/YXW/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,460 words) - 17:14, 25 September 2011
  • Team:Peking R/Notebook/ZMQ/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,450 words) - 17:16, 25 September 2011
  • Team:Peking R/Notebook/MT/zmq
    ...r" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td> <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
    31 KB (4,438 words) - 17:18, 25 September 2011
  • Team:Brown-Stanford/Lab/Notebook/Week6
    *imaged PCR: RBSm|cscB on 7/15, according to 7/11 parameters (may need to remove from thermocycler): *imaged PCR: second round Ana wk/med/str, Nostoc wk (from 7/15)
    5 KB (865 words) - 18:10, 28 September 2011
  • Team:Alberta/Methodology/Notebook/Growth
    <h4>Week 1 May 8 -15</h4> <h4>Week 15 August 15 – 21</h4>
    9 KB (1,453 words) - 16:27, 28 September 2011
  • Summer events/2011 JAPAN Meetup
    |14:30~15:00 |15:00~15:30
    5 KB (661 words) - 19:28, 5 October 2011
  • Team:Calgary/Notebook/Protocols/Process9
    <td>15 μL</td> <td>15 μL</td>
    2 KB (346 words) - 04:26, 29 September 2011
  • Team:Penn/notebook/
    <li>Made 15 1mL-15mL tubes of PBS to use as weights for the centrifuge.</li> <h1>7/15/11</h1>
    70 KB (11,208 words) - 04:45, 29 September 2011
  • Team:Columbia-Cooper/CUNotebook
    <p>Add 1uL ligation to 40uL bacteria incubate on ice for 10-15 min</p> <li> Incubate on ice for 10 - 15 minutes.</li>
    22 KB (3,881 words) - 13:59, 28 October 2011
  • Team:EPF-Lausanne/Notebook/October2011
    * template - 15 uL (for 1.5 ug of DNA, assuming the T7 + Lysis was at a concentration of 10 ...p a protocol (called BLUNTEND) that keeps the tubes at a constant 12 C for 15 minutes. If you want, you can add EDTA to a final concentration of 10 mM (a
    6 KB (1,059 words) - 06:54, 28 October 2011
  • Team:Peking S/lab/notebook/lhc
    |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#7.15|15]] ===7.15===
    15 KB (2,050 words) - 00:17, 6 October 2011
  • Team:Peking S/lab/notebook/dln
    |style="text-align:center"| [[Team:Peking_S/lab/notebook/yrf#7.15|15]] |style="text-align:center"| [[Team:Peking_S/lab/notebook/yrf#8.15|15]]
    12 KB (1,673 words) - 21:59, 5 October 2011
  • Team:SYSU-China/temp/lightbox.js
    ...av-btnPrev').css({ 'background' : 'url(' + settings.imageBtnPrev + ') left 15% no-repeat' }) $(this).css({ 'background' : 'url(' + settings.imageBtnPrev + ') left 15% no-repeat' });
    20 KB (2,138 words) - 07:02, 5 October 2011
  • Team:Kyoto/Cloning
    <li>Dispense 25µL to 15 tubes.</li> # Add 250&micro;L chloroform and shake vigorously for 15 sec.
    11 KB (1,726 words) - 03:39, 5 October 2011
  • Team:Osaka/Notebook/diary
    |<span style={{{s15|"color:{{{c15|red}}};"}}}>15</span> |[[Team:Osaka/week7#September 15 (Thu)|15]]<span style={{{s15|"color:{{{c15|inherit}}};"}}}></span>
    10 KB (1,315 words) - 04:07, 5 October 2011
  • Team:HUST-China/Templates/js3
    ...().hover(function(){a(this).css({background:"url("+p.imageBtnNext+") right 15% no-repeat"})},function(){a(this).css({background:"transparent url("+p.imag
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  • Team:USTC-China/Wet Lab/protocol
    ...d to the center of the plates, which were dried at room temperature at for 15 min, and incubated at 37&deg;C for 10h, and the migration radii were determ ...a(as shown in Figure4.). The plates were dried the room temperature at for 15 min, and incubated at 37&deg;C for 10h, and the migration radii were determ
    7 KB (1,121 words) - 01:55, 29 October 2011
  • Team:USTC-China/Human practice/China Meetup
    </tr><tr><td>14:10-15:25</td> </tr><tr><td>15:25-15:35</td>
    7 KB (1,020 words) - 18:31, 28 October 2011
  • Team:Bielefeld-Germany/Results/S-Layer/CspB CH
    ... After denaturation with 6 M urea the remaining pellet (after centrifuging 15,000 ''g'' for 30 min) was washed with 2 % (v/v) Triton X-100, 2 % SDS (w/v) |style="border-style: solid; border-width: 0 1px 0 0"| 15
    29 KB (4,235 words) - 03:16, 29 October 2011
  • Team:UTP-Panama/After Regional Week 1
    |petri plates (60 x 15 mm) |tubes with SOC media (15 mL)
    5 KB (744 words) - 03:56, 29 October 2011
  • Calendar of Events
    | || '''July 15''' || || [[Jamboree/Project descriptions | Team project descriptions]] due | || '''July 15''' || || [[Jamboree/Safety/Proposals | Safety proposal]] due
    2 KB (275 words) - 21:37, 16 October 2011
  • Regions/Asia/Jamboree/test page
    <li><a href="#15">Software Tools Track Teams</a></li> ...ubmit an abstract of 100-150 words along with your project title (maximum 15 words) by <strong>TBD</strong>. Your abstract will be used in the Jamboree
    19 KB (3,141 words) - 10:16, 28 April 2011
  • Regions/Asia/Jamboree/Pre-Jamboree
    <li><a href="#15">Software Tools Track Teams</a></li> ...ubmit an abstract of 100-150 words along with your project title (maximum 15 words) by <strong>TBD</strong>. Your abstract will be used in the Jamboree
    19 KB (3,134 words) - 01:37, 29 April 2011
  • Team:Greenfield IN-Rihm-HS/Notebook
    <TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">15</font><br><br><br><br></TD> <TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">15</font><br><br><br><br></TD>
    17 KB (2,661 words) - 02:32, 24 June 2011
  • Team:TU-Delft/Team/Tania
    ===Week 15===
    5 KB (891 words) - 15:53, 18 August 2011
  • Team:TU-Delft/Team/Esmee
    ===Week 15===
    5 KB (766 words) - 13:45, 5 July 2011
  • Regions/Europe/Jamboree/During Jamboree
    <li><a class="green" href="#15">Awards</a></li> <li>Aljoscha Wahl +31 15 27 83193</li>
    18 KB (3,104 words) - 09:04, 12 November 2011
  • Team:Michigan/Human Practices
    ==6/15/11==
    7 KB (1,156 words) - 14:39, 27 September 2011
  • Team:Grinnell/Notebook
    ===Week 15-18 Sep 4-Sep 25===
    24 KB (3,886 words) - 03:49, 29 September 2011
  • Team:Michigan/WikiNotebook
    ==6/15/2011==
    1 KB (174 words) - 04:10, 22 June 2011
  • Team:BU Wellesley Software/Notebook/TailiNotebook
    == Week of July 15 (Adding Extended Desktop feature) ==
    9 KB (1,234 words) - 16:46, 27 September 2011
  • Team:BU Wellesley Software/Notebook/MikeyNotebook
    === July 15 ===
    7 KB (1,050 words) - 16:45, 27 September 2011
  • Team:BU Wellesley Software/Notebook/VanessaNotebook
    - More plasmid preps of promoter and RBS from 6/15 transformations. ...lvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h3>Week 6: 7/9-7/15</h3>
    25 KB (3,928 words) - 02:07, 28 September 2011
  • Team:BU Wellesley Software/Notebook/SiruiNotebook
    *'''6/15''': Wellesley lab meeting #2. The team has divided into groups of two to w *'''6/15''': Wet-lab meeting #2 at BU with Suma. The group talked briefly about tra
    29 KB (4,439 words) - 03:57, 29 September 2011
  • Team:Calgary/Notebook/Protocols/Process5
    <li>Split into flasks and autoclave at 121 &deg;C (15 PSI) for 15 minutes </li> ...olution (see below) and autoclave dilute phosphate stock at 121°C (15PSI, 15 mins). After cooling, dispense aseptically with sterilised automatic dispen
    2 KB (258 words) - 04:05, 29 September 2011
  • Team:EPF-Lausanne/Protocols/MITOMI protein DNA
    <p>• flow the expression mix with expressed protein for 10-15 minutes, when ~2-3 cm of mix left in the inlet tube :</p>
    2 KB (386 words) - 08:49, 15 July 2011
  • Team:BU Wellesley Software/Notebook/ChenkaiNotebook
    <h2>Wednesday 6/15</h2>[[File: RMDebuggerScreenShot.png‎ |500px|thumb|right|Debugger printou <h2>Friday 7/15</h2>
    28 KB (4,611 words) - 16:34, 27 September 2011
  • Team:Michigan/DryLabNotebook
    ==6/15/2011==
    454 B (66 words) - 21:53, 6 July 2011
  • Team:Edinburgh/tech/basiccss
    z-index: 15; z-index: 15;
    5 KB (574 words) - 22:58, 25 October 2011
  • Team:Caltech/Week 1
    ==6/15==
    348 B (54 words) - 08:33, 20 June 2011
  • Team:Brown-Stanford/Lab/Notebook/Week0
    == '''June 15 2011''' ==
    6 KB (905 words) - 04:16, 29 September 2011
  • Reporter: Week 7 June 26-July 1
    |0:15 |1:15
    11 KB (1,693 words) - 17:33, 5 July 2011
  • Team:Caltech/Week 6
    ...n, 11 pNT001 10 min, 12 pNT001 15 min, 13 pNT001 30 min, 14 pNT001 60 min, 15 NEB 2-log ladder. Triangles represent increasing time (not to scale)]]
    6 KB (926 words) - 22:19, 26 July 2011
  • Team:UANL Mty-Mexico
    padding-top: 15; padding-right: 15;
    22 KB (2,497 words) - 16:35, 13 February 2012
  • Team:Waterloo
    ...up I introns ribozyme. Graduate School of Biostudies, Kyoto University. 30(15): 3473-3480. ...="https://static.igem.org/mediawiki/2011/b/b0/Screen_Shot_2011-09-29_at_12.15.05_AM.png" alt="Peter", width="160" height="200">Peter Hong</img></div>
    132 KB (19,352 words) - 19:19, 28 October 2011
  • Si Chen's Lab Notebook
    [[15]] [[15]]
    4 KB (397 words) - 18:13, 2 July 2011
  • Nina Jiauye Zhu's Lab Notebook
    [[15]] [[15]]
    4 KB (397 words) - 20:16, 2 July 2011
  • Yuanwei Li's Lab Notebook
    [[15]] [[15]]
    5 KB (404 words) - 21:58, 2 July 2011
  • Team:British Columbia/Notebook/15 June 2011
    ==June 15 2011==
    93 B (15 words) - 04:00, 4 July 2011
  • Team:Brown-Stanford/Lab/Protocols/RegistryCompetency
    * Add glycerol to 15% ** Transformation efficiency is (dilution factor=15) x colony count x 10<sup>5</sup>/µgDNA
    7 KB (1,045 words) - 18:18, 28 September 2011
  • Team:Edinburgh/Experiments
    :: 900 plate> 15 white, 4 blue :: 100 plate> 15 white
    15 KB (2,228 words) - 19:59, 28 October 2011
  • Team:Caltech/Week 5
    ==July 15==
    6 KB (1,056 words) - 18:25, 20 July 2011
  • Team:Nairobi/Safety
    ...terization. Journal of Investigational Allergology and Clinical Immunology 15:131_139. ...terization. Journal of Investigational Allergology and Clinical Immunology 15:131_139.
    23 KB (3,299 words) - 19:22, 5 September 2011
  • Team:Wisconsin-Madison/notebookprotocols
    </li><li> Repeat steps 14 and 15 to remove all traces of isopropanol. Pulse spin after removing bulk of fina </li><li> Air dry the pellet for ~15 minutes (pellet will change from white to clear as it dries). Resuspend in
    24 KB (3,605 words) - 16:25, 8 August 2011
  • Team:Freiburg/Notebook/22 July
    || <div align="right">1.15</div> *meeting for the wiki takeover: wednesday 9:00 am (new time: 12:15)
    16 KB (2,075 words) - 00:59, 22 September 2011
  • Team:Harvard/Results
    ...o optimizing our selection system, we were able to test and discover up to 15 novel zinc fingers that potentially binds to the colorblindness gene target ...s on the chip that was submitted, meaning that '''we have discovered up to 15 novel zinc fingers'''. The sequences consistent with the chip are CBBot_A1,
    6 KB (957 words) - 21:59, 28 October 2011
  • Team:Alberta/Methodology/Protocols
    <li>For solid PD agar, add 15 g of agar per 1 L of media.</li> <td>15 g</td>
    18 KB (2,545 words) - 00:27, 29 September 2011
  • Team:Cornell/Week 6
    ==Friday, July 15==
    3 KB (437 words) - 17:22, 18 September 2011
  • Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification
    <b>3.</b> Harvest the bacterial cells by centrifugation at 6000 x g for 15 min at 4°C. ...sly inverting the sealed tube 4-6 times, and incubate at room temperature (15-25°C) for 5 min.
    7 KB (990 words) - 10:44, 10 January 2012
  • Team:Cambridge/Experiments/Squid Dissection and Tissue Sample
    !'''15''' !'''15'''
    14 KB (2,139 words) - 01:43, 22 September 2011
  • Team:Cornell/Week 11
    ==Monday, August 15==
    7 KB (954 words) - 17:23, 18 September 2011
  • Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2 trans
    <b>2.</b> Aliquot the culture into fractions of the desired volume V ( 10 or 15 mL usually ). <b>9.</b> Empty the supernatant and resuspend ( do not vortex ) in V/15 mL CaCl2 and V/30 mL glycerol 40%
    3 KB (452 words) - 10:18, 10 January 2012
  • Team:UANL Mty-Mexico/Templates/Menu-template
    padding-top: 15; padding-right: 15;
    17 KB (1,789 words) - 16:38, 13 February 2012
  • Team:Freiburg/Notebook/15 August
    <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 15 August </a> 72° 1'15'' <br/>
    6 KB (735 words) - 01:08, 22 September 2011
  • Team:Freiburg/Notebook/16 August
    ===2A Assembly like August 15===
    9 KB (1,123 words) - 01:09, 22 September 2011
  • Team:Freiburg/Notebook/22 August
    || <div align="right">0:15</div> || <div align="right">0:15</div>
    17 KB (1,983 words) - 01:11, 22 September 2011
  • Team:Freiburg/Notebook/30 August
    Aliquot 15 μl and store at -20 °C. This assembly mixture can be stored at -20 °C fo 3. Thaw a 15 μl assembly mixture aliquot and keep on ice until ready to be used.
    25 KB (2,824 words) - 01:13, 22 September 2011
  • Sensor: Week 9 July 12-15
    ==Friday, July 15==
    2 KB (254 words) - 20:01, 30 July 2011
  • Team:Caltech/Week 8
    ...r, 2-4 pNT003 insert, 5-7 pNT003 insert with PIPE primers, 8-13 pSB3K3, 14-15 pETDEST53 ... PIPE primers, 8-9 pNT003 insert, 10-11 pNT003 inset with PIPE primers, 12-15 pETDEST53
    6 KB (927 words) - 17:19, 9 August 2011
  • Reporter: Week 9 July 10-16
    ==Friday, July 15==
    5 KB (819 words) - 15:21, 1 August 2011
  • Team:Paris Liliane Bettencourt/Notebook/2011/08/01/
    * Primer fwd ( 1μM = 15,18 ng/μL) : 8,2 μL (125ng) * Primer rev ( 1μM = 15,18 ng/μL) : 8,2 μL (125 ng)
    3 KB (518 words) - 16:34, 1 September 2011
  • Team:Imperial College London/Extras/Brainstorming/Health test
    ...cer cells) - Prodigiosin biosynthesis gene cluster (pig cluster) contains ~15 ORFs in Serratia strains (Harris et al., 2004) - PigS and PigP regulate pro ...pes of animals. One of the most common venoms are produced by snakes where 15% of 3,000 species of snakes are found poisonous. Snake venom consists of pr
    28 KB (4,593 words) - 09:30, 8 August 2011
  • Team:Harvard/Template:NotebookData4
    **annealing temp 65˚C, elongation time 1:15 *10 cycles without primers; hisura-kan_F and ZFB-wp-hisura_R primers added; 15 more cycles
    31 KB (4,866 words) - 16:56, 8 August 2011
  • Team:Imperial College London/Project/Auxin/Results
    ... 11- 7a cut with E; Lane 12- 7a cut with P; Lane 13- 7a cut with E+P; Lane 15- 7b cut with E; Lane 16- 7b cut with P; Lane 17- 7b cut with E+P. Gel 2: La ...ladder; lanes 2 to 7 - PCR of vector 6 from 57.1°C to 62.6°C; lanes 9 to 15 - PCR of vector 7 from 57.1°C to 62.6°C. Gel 4: lanes 2 to 7 - PCR of vec
    24 KB (4,082 words) - 16:03, 12 September 2011

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