Team:TU-Delft/Notebooks/Protocols/LBMedium

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==='''LB Medium 1 liter'''===
==='''LB Medium 1 liter'''===
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1. Measure 950 ml of demi water and put it in a flask
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1. Measure 950 ml of demi water and put it in a flas
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2. Add 10 grams of NaCl
2. Add 10 grams of NaCl
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3. Add 10 grams of Bacter Tryptone
3. Add 10 grams of Bacter Tryptone
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4. Add 5 grams of Yeast extract
4. Add 5 grams of Yeast extract
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5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH
5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH
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6. Fill up to 1 liter
6. Fill up to 1 liter
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7. Bring the flask to the autoclave
7. Bring the flask to the autoclave
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[https://2011.igem.org/Team:TU-Delft/Notebooks/Protocols Back to Protocols...]
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Latest revision as of 12:51, 9 August 2011



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Protocol

LB Medium 1 liter

1. Measure 950 ml of demi water and put it in a flas

2. Add 10 grams of NaCl

3. Add 10 grams of Bacter Tryptone

4. Add 5 grams of Yeast extract

5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH

6. Fill up to 1 liter

7. Bring the flask to the autoclave

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