Team:UEA-JIC Norwich/Methods

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Latest revision as of 21:18, 21 September 2011

University of East Anglia-JIC

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+<h1>Click on the happy scientist to open the protocol
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+<Br>
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+High Efficiency Transformation Protocol
-#menubar.left-menu a {color:white;}
+<br>
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+[[File:Happy_scientist.gif|High Efficiency Transformation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/transformation]]
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+<br>
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+Culture Preparation Protocol
+<br>
-#headerimage {width:965px; margin-bottom:10px}
+[[File:Happy_scientist.gif|Culture Preparation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/cultureprep]]
-#headerimage img {max-width:965px;}
+<br>
-#headermenu {text-align:center; width:965px; height:22px;border-bottom:1px solid #529bc7;}
+Glycerol Stock Solution Protocol
-#headermenu ul {display:inline-block; zoom:1;}
+<br>
-#headermenu ul.top {margin-left:0;}
+[[File:Happy_scientist.gif|Glycerol Stock Solution Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/glycerolstock]]
-#headermenu ul {text-align:left; margin:0; padding:0; list-style:none; white-space:nowrap;}
+<br>
-#headermenu li {margin:0; padding:0;}
+Qiagen Miniprep Protocol
-#headermenu a {display:block; font:normal 11px verdana,arial,sans-serif;color:#82CAFA; line-height:22px; text-decoration:none; padding:0 20px;}
+<br>
-#headermenu li:hover > ul {visibility:visible;}
+[[File:Happy_scientist.gif|Qiagen Miniprep Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/miniprep]]
-#headermenu a:hover ul,
+<br>
-#headermenu a:hover a:hover ul,
+Gel Electrophoresis Protocol
-#headermenu a:hover a:hover a:hover ul {visibility:visible;}
+<br>
-#headermenu a:hover ul ul,
+[[File:Happy_scientist.gif|Gel Electrophoresis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/electrophoresis]]
-#headermenu a:hover a:hover ul ul {visibility:hidden;}
+<br>
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+PCR Protocol
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+<br>
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+[[File:Happy_scientist.gif|PCR Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pcr]]
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+<br>
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+PEG Transformation Protocol
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+<br>
-#headermenu a.top-a:hover b,
+[[File:Happy_scientist.gif|PEG Transformation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pegtransformation]]
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+<br>
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+Algae Transformation using glass beads
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+<br>
-#headermenu a.down:focus b,
+[[File:Happy_scientist.gif|Algae Transformation using glass beads Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeglassbeads]]
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+<br>
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+Algae Transformation using electroporation
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+<br>
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+[[File:Happy_scientist.gif|Algae Transformation using electroporation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeelectroporation]]
-#headermenu li ul {display:block; position:absolute; visibility:hidden; background:#529bc7; padding:1px 1px 8px 1px; left:0;}
+<br>
-#headermenu li li {border-bottom:1px solid #529bc7;}
+Site Directed Mutagenesis Protocol
-#headermenu li li a {background:#fff;}
+<br>
-#headermenu li li a:hover {background:#153E7E;}
+[[File:Happy_scientist.gif|Site Directed Mutagenesis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/sdm]]
-#headermenu li li:hover > a {background:#153e7e;}
+<br>
-#headermenu ul.drop-down {top:22px; opacity:1.0;}
+pGEM T-easy Vector Cloning protocol
-#headermenu li li ul {left:100%; margin-top:-23px; margin-left:-5px;}
+<br>
-#headermenu table {text-align:left; position:absolute;top:0;left:0;border-collapse:collapse;}
+[[File:Happy_scientist.gif|pGEM T-easy Vector Cloning Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/vectorcloning]]
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+<br>
-#headermenu table table {top:auto; left:100%; margin-left:-1px; padding:0; margin:0;}
+Restriction Digest protocol
-#headermenu table table ul {margin-top:-4px; marg\in-top:-7px;}
+<br>
-</style>
+[[File:Happy_scientist.gif|Restriction Digest Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/rdigest]]
-<div id="wrap">
+<br>
-<div id="headermenu">
+Poly "A" Tailing Protocol
-	<ul class="top">
+<br>
-		<li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich"><b>Home</b></a>
+[[File:Happy_scientist.gif|Poly "A" Tailing PCR Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/polyatailing]]
-</li>
+<br>
+Ligation Protocol
-		<li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Team"><b>Team</b></a>
+<br>
+[[File:Happy_scientist.gif|Ligation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/ligation]]
-		</li>
+<br>
+Gel Extraction Protocol
-		<li class="top-li"><a class="top-a down" href=""><b>Aims</b>
+<br>
-			<ul class="drop-down">
+[[File:Happy_scientist.gif|Gel Extraction Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/gelextract]]
-				<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Project">Overview</a></li>
-				<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Nittygritty-bacteria">Bacteria</a></li>
-				<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Nittygritty-algae">Algae</a></li>
-                                <li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Nittygritty-moss">Moss</a></li>
-			</ul>
-		</li>
-		<li class="top-li"><a class="top-a down" href=""><b>Registry Parts</b>
-			<ul class="drop-down">
-				<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Registryoverview">Overview</a></li>
-				<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Registrycharacterisation">Characterization</a></li>
-			</ul>
-		</li>
-<li class="top-li"><a class="top-a down" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Methods"><b>Methods</b>
-		</li>
-		<li class="top-li"><a class="top-a down" href=""><b>Journal</b>
-			<ul class="drop-down">
-				<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Notebook">Lab Journal</a></li>
-				<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/DaytoDay">Day to Day Journal</a></li>
-			</ul>
-		</li>
-	<li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Safety"><b>Lab Safety</b></a>
-</li>
-<li class="top-li"><a class="top-a down" href=""><b>Human Practices</b></a>
-<ul class="drop-down">
-				<li><a href="top-a down" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Outreach">Outreach</a></li>
-				<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Energyconservation">Energy Conservation</a></li>
-			</ul></li>
-  <li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Media"><b>Media</b></a>
-</li>
-<li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Follow_us"><b>Follow Us</b></a>
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-<h2>High Efficiency Transformation Protocol</h2>
-<p>1. Thaw a tube of NEB 5-alpha Competent E.coli cells on ice for 10 minutes</p>
-<p>2. Mark the location of the Biobrick well (letters from top to bottom, numbers from left to right)</p>
-<p>3. Resuspend specific biobrick part (1 μl) with distilled water (20 μl). Aspirate up and down a few times</p>
-<p>4. Inoculate with DNA ( 1 μl) to the competent cells</p>
-<p>5. Place the mixture on ice for 30 minutes. Do not mix</p>
-<p>6. Heat shock at exactly 42°C for exactly 30 seconds. Do not mix</p>
-<p>7. Place on ice for 5 minutes. Do not mix</p>
-<p>8. Pipette 950 μl of room temperature SOC into the mixture</p>
-<p>9. Place at 37°C for 60 minutes. Shake vigorously (250 rpm) or rotate</p>
-<p>10. Warm selection plates to 37°C</p>
-<p>11. Do serial dilutions (2-3) at 105. Mix cells, flick/invert gently</p>
-<p>12. Spread (100 μl) on agar plates of each dilution</p>
-<p>13. Incubate overnight at 37°C</p>
-<h2>Culture Preparation Protocol</h2>
-<p>1. Select a colony by sampling it with a wooden pick</p>
-<p>2. Place into the appropriate antibiotic LB broth</p>
-<p>3. Incubate at 37°C overnight in an Incubator Shaker</p>
-<p>4. Store at -20°C</p>
-<h2>Glycerol Stock Solution Protocol</h2>
-<p>1. Take 500µL of a previously prepared culture and place into a screw-top eppendorf tube</p>
-<p>2. Add 500µL of Glycerol solution (V.V 50% to make a 25% concentration</p>
-<p>3. Place in -20°C storage</p>
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