Team:UEA-JIC Norwich/Methods

From 2011.igem.org

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Latest revision as of 21:18, 21 September 2011

University of East Anglia-JIC

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-<div style="background-color:#6AFB92; color:#254117">
+{{Banner}}
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+[[file:Research_methodsbanner.jpg|center]]
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+<br>
-<style type="text/css">
+<br>
-ul
+<p style="color:#000000">
-{
+<center>
-list-style-type:none;
+<h1>Click on the happy scientist to open the protocol
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+</h1>
-padding:0;
+<Br>
-padding-top:6px;
+High Efficiency Transformation Protocol
-padding-bottom:6px;
+<br>
-}
+[[File:Happy_scientist.gif|High Efficiency Transformation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/transformation]]
-li
+<br>
-{
+Culture Preparation Protocol
-display:inline;
+<br>
-}
+[[File:Happy_scientist.gif|Culture Preparation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/cultureprep]]
-a:link,a:visited
+<br>
-{
+Glycerol Stock Solution Protocol
-font-weight:bold;
+<br>
-color:#254117;
+[[File:Happy_scientist.gif|Glycerol Stock Solution Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/glycerolstock]]
-text-align:center;
+<br>
-padding:6px;
+Qiagen Miniprep Protocol
-text-decoration:none;
+<br>
-text-transform:uppercase;
+[[File:Happy_scientist.gif|Qiagen Miniprep Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/miniprep]]
-}
+<br>
-a:hover,a:active
+Gel Electrophoresis Protocol
-{
+<br>
-background-color:#387C44;
+[[File:Happy_scientist.gif|Gel Electrophoresis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/electrophoresis]]
-}
+<br>
+PCR Protocol
-</style>
+<br>
-</head>
+[[File:Happy_scientist.gif|PCR Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pcr]]
+<br>
-<body>
+PEG Transformation Protocol
-<ul>
+<br>
-<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich">Home</a></li>
+[[File:Happy_scientist.gif|PEG Transformation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/pegtransformation]]
-<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Team">The Team</a></li>
+<br>
-<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Project">Aims</a></li>
+Algae Transformation using glass beads
-<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Parts">Parts Submitted To The Registry</a></li>
+<br>
-<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Methods">Methods</a></li>
+[[File:Happy_scientist.gif|Algae Transformation using glass beads Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeglassbeads]]
-<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Notebook">Lab Journal</a></li>
+<br>
-<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Safety">Lab Safety</a></li>
+Algae Transformation using electroporation
-<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Blog">Follow Us</a></li>
+<br>
-<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Blog">Outreach</a></li>
+[[File:Happy_scientist.gif|Algae Transformation using electroporation Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/algaeelectroporation]]
-</ul>
+<br>
-</body>
+Site Directed Mutagenesis Protocol
+<br>
-<h2>High Efficiency Transformation Protocol</h2>
+[[File:Happy_scientist.gif|Site Directed Mutagenesis Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/sdm]]
+<br>
-Perform steps 1-13:
+pGEM T-easy Vector Cloning protocol
-<ol>
+<br>
-#Thaw a tube of NEB 5-alpha Competent E.coli cells on ice for 10 minutes.
+[[File:Happy_scientist.gif|pGEM T-easy Vector Cloning Protocol |link=https://2011.igem.org/Team:UEA-JIC_Norwich/vectorcloning]]
-#Mark the location of the Biobrick well (letters from top to bottom, numbers from left to right).
+<br>
-<li>Resuspend specific biobrick part (1 μl) with distilled water (20 μl). Aspirate up and down a few times.</li>
+Restriction Digest protocol
-<li>Inoculate with DNA ( 1 μl) to the competent cells.</li>
+<br>
-<li>Place the mixture on ice for 30 minutes. Do not mix.</li>
+[[File:Happy_scientist.gif|Restriction Digest Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/rdigest]]
-<li>Heat shock at exactly 42°C for exactly 30 seconds. Do not mix.</li>
+<br>
-<li>Place on ice for 5 minutes. Do not mix.</li>
+Poly "A" Tailing Protocol
-<li>Pipette 950 μl of room temperature SOC into the mixture.</li>
+<br>
-<li>Place at 37°C for 60 minutes. Shake vigorously (250 rpm) or rotate.</li>
+[[File:Happy_scientist.gif|Poly "A" Tailing PCR Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/polyatailing]]
-<li>Warm selection plates to 37°C.</li>
+<br>
-<li>Do serial dilutions (2-3) at 105 . Mix cells, flick/invert gently.</li>
+Ligation Protocol
-<li>Spread (100 μl) on agar plates of each dilution.</li>
+<br>
-<li>Incubate overnight at 37°C.</li>
+[[File:Happy_scientist.gif|Ligation Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/ligation]]
-</ol>
+<br>
+Gel Extraction Protocol
+<br>
+[[File:Happy_scientist.gif|Gel Extraction Protocol|link=https://2011.igem.org/Team:UEA-JIC_Norwich/gelextract]]
-</html>