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Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
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REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
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Lab
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Partners
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Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
Protocols
Media
- M1 - BG-11
- M2 - LB (Lysogeny broth)
- M3 - ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- M4 - Enrichment media
- M5 - Nutrient agar with urea
- M6 - SOB (Super Optimal Broth) and SOC (Super Optimal Broth with Catabolite repression)
- M7 - Bang Media
- M8 - Urease Test Plates
S. pasteurii Transformation Media
Biobrick Constructions
Competency and Transformations
- CT1 - Registry Distribution Transformation
- CT2 - Registry Distribution Competency
- CT3 - CaCl2 Competency
- CT4 - Fast CaCl2 Combined Competency and Transformation
- CT5 - Protoplast Transformation
- CT6 - Conjugal transfer into Anabaena
- CT7 - NEB 5-alpha High-Efficiency Transformation
Assembly
