Protocol Code: M3
ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- 20.0 g Yeast extract
- 10.0 g (75.7 mM) (NH4)2SO4
- 1.0 L 0.13 M Tris buffer (pH 9.0)
- 20.0 g Agar (if needed)
Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.