Team:Brown-Stanford/Lab/Protocols/ATCC1376

From 2011.igem.org

Brown-Stanford
iGEM

Protocol Code: M3

ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)

  • 20.0 g Yeast extract
  • 10.0 g (75.7 mM) (NH4)2SO4
  • 1.0 L 0.13 M Tris buffer (pH 9.0)
  • 20.0 g Agar (if needed)

Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.