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Team:Brown-Stanford/Lab/Protocols/CaCl
From 2011.igem.org
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Protocol Code: CT3
CaCl
2
Competency
Buffers
TfBI
30mM KOAc
50mM MnCl
2
-4H
2
O
100mM KCl
10mM CaCl
2
-2H
2
O
15% Glycerol
TfBII
10mM Na-MOPS pH 7
75mM CaCl
2
10mM KCl
10% Glycerol